For Table 1:calculate the [PNP] in μM for each of these samples.For Table 2:Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations. (WATCH YOUR UNITS OF MEASUREMENT)Buffer(μl)[PNP] in uM150014901480147014605145061440775142581001400Note that in the PNP column, uM = μmoles/liter.Table 1Tube #Blank123+104PNP (μl)0.5 mM stock0102030405060Trial 1A405Trial 2A405 Table 2Tube #Blank12345PNPP (ul)100 mM stock0Buffer (μl)1000Enzyme(μl)055555Final volume(μl)100010001000100010001000[PNPP] in uM02004006008001000

0.5mM =0.5 ×10-3 moles literi.e. 1 liter have 0.5 ×10-3 molesSo, 106 μL have 0.5 ×10-3 molesSo, 1…

calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.

calculate the [PNP] in μM for each of these samples. For Table 2: Calculate the volumes of the 100 mM PNPP stock solution and buffer (final volume of 1.00 ml) needed to get each of the substrate concentrations.

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Question

For Table 1:

calculate the [PNP] in μM for each of these samples.

For Table 2:

Calculate the volumes of the 100 mM PNPP stock solution and buffer (final
volume of 1.00 ml) needed to get each of the substrate concentrations.

(WATCH YOUR UNITS OF MEASUREMENT)
Buffer
(μl)
[PNP] in uM
1500
1490
1480
1470
1460
5
1450
6
1440
7
75
1425
8
100
1400
Note that in the PNP column, uM = μmoles/liter.
Table 1
Tube #
Blank
1
2
3
+10
4
PNP (μl)
0.5 mM stock
0
10
20
30
40
50
60
Trial 1
A405
Trial 2
A405

Table 2
Tube #
Blank
1
2
3
4
5
PNPP (ul)
100 mM stock
0
Buffer (μl)
1000
Enzyme
(μl)
0
5
5
5
5
5
Final volume
(μl)
1000
1000
1000
1000
1000
1000
[PNPP] in uM
0
200
400
600
800
1000

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