Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 mL x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) In % Ingredients In Grams/300mL Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As needed

Human Anatomy & Physiology (11th Edition)
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Author:Elaine N. Marieb, Katja N. Hoehn
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Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non-
fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare
300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your
calculations.
*A sample calculation was made for you.
Peptone = 300 ml x 0.5% = 1.5 g
Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019)
Ingredients
In %
In Grams/300ml
Peptone
0.5
*1.5
Meat extract
0.3
Agar
1.5
Distilled Water
As needed
Transcribed Image Text:Nutrient Agar (NA) is a general purpose medium used for the cultivation of a wide variety of non- fastidious microorganisms (Merck, 2000). Its ingredients are listed in Table 2. You are tasked to prepare 300ml of NA, determine the amount of ingredients and write in column 3 in Table 2. Show your calculations. *A sample calculation was made for you. Peptone = 300 ml x 0.5% = 1.5 g Table 2. Medium Composition of Nutrient Agar (NA) with the recommended proportions (Merck, 2019) Ingredients In % In Grams/300ml Peptone 0.5 *1.5 Meat extract 0.3 Agar 1.5 Distilled Water As needed
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