You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.

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You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to
plate out 100 cells in a volume of 200 μL. Explain how you would do this. 

Expert Solution
Step 1

We shall be performing a dilution calculation for this question.

We know, C1V1 = C2V2

  • C1 is the concentration of the stock solution.
  • V1 is the volume to be aliquoted from the stock.
  • C2 is the final concentration of the diluted solution.
  • V2 is the final volume of the diluted solution.
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