You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.
You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to plate out 100 cells in a volume of 200 μL. Explain how you would do this.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have a culture of cells at a density of 2.7 x 109 cells/mL and you want to
plate out 100 cells in a volume of 200 μL. Explain how you would do this.
Expert Solution
Step 1
We shall be performing a dilution calculation for this question.
We know, C1V1 = C2V2
- C1 is the concentration of the stock solution.
- V1 is the volume to be aliquoted from the stock.
- C2 is the final concentration of the diluted solution.
- V2 is the final volume of the diluted solution.
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