1.)the order of steps done for immunoprecipitation is a.) 1° Ab, 2° Ab, SDS-PAGE b.)SDS-PAGE, 1 °Ab, 2° Ab c.)1° Ab, SDS-PAGE, 2° Ab d.)SDS-PAGE, 2° Ab, 1°Ab

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Problem 2(Classwork)
1. )the order of steps done for immunoprecipitation is
a.) 1° Ab, 2° Ab, SDS-PAGE b.)SDS-PAGE, 1 °Ab, 2° Ab
c.)1° Ab, SDS-PAGE, 2° Ab d.)SDS-PAGE, 2° Ab, 1°Ab
2)similarity between Western blot and ELISA is
a.)both have two 1° Ab to protein of interest b.)both use SDS-PAGE c.)both use
centrifugation d.)both detect protein of interest by measure of product made by enzyme
3.)the second antibody added in ELISA has this property
a.)it is bound to a large, heavy molecule b.)it is added after SDS-PAGE
C. it recognizes primary antibody d.)it recognizes protein of interest
Transcribed Image Text:Problem 2(Classwork) 1. )the order of steps done for immunoprecipitation is a.) 1° Ab, 2° Ab, SDS-PAGE b.)SDS-PAGE, 1 °Ab, 2° Ab c.)1° Ab, SDS-PAGE, 2° Ab d.)SDS-PAGE, 2° Ab, 1°Ab 2)similarity between Western blot and ELISA is a.)both have two 1° Ab to protein of interest b.)both use SDS-PAGE c.)both use centrifugation d.)both detect protein of interest by measure of product made by enzyme 3.)the second antibody added in ELISA has this property a.)it is bound to a large, heavy molecule b.)it is added after SDS-PAGE C. it recognizes primary antibody d.)it recognizes protein of interest
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