Why should agar media be completely dissolved before they are dispensed in tubes and plates? What are the bases for pegging the temperature at 1210C for 15-30 minutes during moist heat sterilization and 1800C for two (2) hours using dry heat sterilization? Can you sterilize culture media using dry heat sterilization? Why is that so? You will notice in the videos shown, the cotton plug is not used. What is role of cotton plug in media prep, sterilization and culture of microorganisms?       Instead of using cotton plug, plastic screw-cap is used, can you substitute this for the former? Is it technically acceptable in microbiology?

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: How would you prepare a 10-7dilution from a culture? Each of your dilution tubes cannot hold more…

A: Dilution is the process of lowering a solute's concentration in a solution, typically by mixing it…

Q: There are two cultures of yeast cells in the pictures, one has been incubated for 6 hours and one…

A: Let's say, the yeast cell culture which was incubated for 6  hours as  = sample A the yeast cell…

Q: What would your final dilution be if you transferred 0.1 mL of a 10-6 dilution and added it to a…

A: The pour plate method is a technique used to count the number of colony-forming bacteria found in a…

Q: Why do cells need to be kept in the freezing devices first then transferred to liquid nitrogen…

A: Rapid freezing of cells can cause ice crystals large crystals can be formed in which cells are…

Q: Compare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in…

A: Introduction Sterilization is the process of completely destroying or eliminating all forms of…

Q: What are some methods to prevent contamination during streak plating a medium?

A: Streak plate method is one of the most important methods used by the laboratories to isolate single…

Q: Why is it sufficient to stain cells from a SDA (Sabouraud Dextrose Agar) plate with crystal violet…

A: Introduction : Sabouraud agar, often known as Sabouraud dextrose agar (SDA), is a peptone-containing…

Q: Agar is a solidifying agent used in media preparation. What is its origin?

A: Agar gel is a polysaccharide which can become liquefied at temperatures ranges from 85°C to 90°C and…

Q: Assume that you are adding 300 microliters of 1% substrate solution per well in a 24-well plate. If…

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Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: Questions 1 Predict the effect of the following "mistakes" made when performing a Gram stain. Your…

A: In order to understand the mistakes made in Gram staining procedure we need to understand the whole…

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…

A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…

Q: You are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL.…

A: We are given a bacterial culture containing approximately 5.0 x 108 cells/mL. To identify the exact…

Q: A culture medium is comprised of the following components: 0.5% peptone, 0.3% meat extract, 1.5%…

A: In this scenario, we are tasked with preparing a culture medium with specific components, including…

Q: Can you please discuss the principles of using Motility Test Medium for the detection of bacterial…

A: Bacterial motility is usually demonstrated in a semi-solid agar medium. Motile bacteria ‘swarm' in…

Q: You aseptically transfer 1 ml of your optional liquid culture into 99 ml of sterile water. What is…

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Q: What nutrients do the following media components contain?   Peptone   Yeast extract   Beef…

A: “Since you have posted a question with multiple sub-parts, we will solve the first three sub-parts…

Q: What would happen if an ampicillin reagent is added to an Lysogeny broth agar plate that is below 50…

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Q: where 5 nutrient agar pates per female student and 3 agar slants per males student are needed to…

A: It is given that 5 nutrient agar plate prepared by per female so Seven female will prepare=7*5=35…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: Why is PBS buffer preferred when preparing a monolayer cell culture?

A: PBS (phosphate buffer saline) is a buffer solution which is a water based salt solution containing…

Q: What is the amount (mL) of pre-culture (108 cell/mL) necessary to inoculate (to add) in a 2-liter…

A: Culture media is a solid or semisolid substance used to support the growth of microorganisms. It is…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Q: The students of a Microbiology class were tasked to transfer or subculture a pure culture of…

A:

Q: You have a broth medium that you would like to turn into a solid medium in order to do streak…

A: The "nutrient broth" is essentially nutrient agar without the solidifying agent. They stay liquid at…

Q: Media is TGA explaining based on what kind of microorganisms it allows to grow?

A: TGA is a complex media based upon its chemical composition. Complex media are the media in which the…

Q: If you prepare culture media plates 2 days ahead of the laboratory schedule, can you still use the…

A: Preparation of culture media and inoculation are two of the very sensitive steps in any…

Q: What is differential medium

A: Differential medium or indicative media contain compounds which support the growth of microbes and…

Q: What is the name of this media? What type of media is it? What does the yellow color indicate? What…

A: Selective media generally selects for the growth of a desired organism, stopping the growth of or…

Q: What is the advantage of the plating method over an electronic cell counting method in counting…

A: Bacteria culturing is done in order to grow the bacterial strains that are able to show resistance…

Q: What is the purpose of adding sodium bicarbonate to a culture medium?

A: >> The purpose of adding sodium bicarbonate to animal culture medium is that it acts as a…

Q: For the following agar media used, list their significant components and explain how they react.…

A: The sheep blood agar is a popular agar medium. It is a differential medium that includes important…

Q: WHY IN INDOLE TEST A COLORED RING FORM AT THE TOP OF THE CULTURE MEDIA BUT NOT THE WHOLE CULTURE…

A: Indole test is a biochemical test for distinguishing between Enterobacter aerogenes and Escherichia…

Nutrient Growth Medium

It is a culture medium or nutrient broth for all kinds of microorganisms to grow properly and create their colony. This matrix provides support to increase the population of microbes through the method of cell proliferation. It is regarded as the food material for those organisms. It is a type of in vitro mechanism, in which the development of life is processed outside the host body. It can be solid, semi-solid, or liquid in nature. Different kinds of cells develop in different types of media.