e.2. What is the amount of each component needed, given the following media composition per liter of distilled water: computation Show your Component g/L tryptone 10.0 NaCI 5.0 yeast extract 10.0 agar 15.0
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- How do eosin-methylene blue (EMB) agar plates work? What organism(s) are they designed to detect? How would a positive test appear on the plate?For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. 1. What is the total volume of the medium needed? Show your computationA sample of water was enumerated using a counting chamber (haemocytometer) and 220 bacterial cells were counted in 25 small squares (each of volume 2.5 x 10-7 cm3). Viable counts were carried out on the same culture using both the pour plate method (incorporating 1 mL samples of a range of dilutions into plate count agar plates) and the Miles & Misra spread plate method, where 0.02 mL samples were spread on sectors of a plate count agar plate. For the pour plate count the average of triplicate samples was 178 colonies per plate of the 10-4dilution. For the Miles and Misra count the average of triplicate samples was 21.3 colonies per sector of the 10-4 dilution. Calculate the total count and the viable pour plate and spread plate counts and suggest possible reasons for the difference between the three different counts.
- A student needed to transfer bacteria from a broth culture to an agar plate. Below is the step-by-step what was done to accomplish this. The transfer of bacteria was not successful because of which step? 1. Cap of the broth culture is removed 2. The mouth of the bottle is flamed 3. The loop was flamed 4. The loop was inserted into the culture to pick up the bacteria 5. The loop was flamed 6. The loop containing the bacteria was used to introduced to spread the agar plate 7. The plate was placed in an incubator at 30 CelciusWhile conducting the Hydrogen Sulfide Test (SIM Agar) you open up one of the tubes and it gives off a rotten egg smell while another tube you cultured does not. What does the smell indicate? How come there was no change in color in the tube that indicated a positive test? Please explain how the color change is to occur. Think critically.Which of the following is the proper technique for inoculating an agar slant with a broth culture? A. Stab the butt of the media with the wire loop. B. Stab the surface of the agar media with the wire loop beginning at the base of the tube moving toward the mouth as you withdraw the loop. C. Gently move the wire loop back and forth across the surface of the agar beginning at the mouth of the tube moving down toward the base of the slant. D. Gently move the wire loop back and forth across the surface of the agar beginning at the base of the slant as you withdraw it from the tube.
- What test can help you differentiate staphylococci from streptococci? What reagent or media would be use? short answer onlyYou are given a bacterial culture which has a concentration of approximately 5.0 x 10^8 cells/mL. List a series of dilutions and platings that you could carry out in order to determine the exact concentration of the culture. Note that you must plate four plates from a minimum of two dilution tubes. The volumes plated should be in the range of 0.1 mL – 1.0 mL. Duplicate volumes may not be plated from any one dilution tube. Each plating should aim for a count between 30 and 300 CFUs. You can select any value from 30-300 for CFU and any volume from 0.1-1.0 to find out dilution schemeYou are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method what other methods could you use to separate the bacteria? Please state what method(s) you would use, the result you would be looking for to help identify each bacterium and the interpretation of the result.
- Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase TestAndrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?