To quantify the transcription product of a specific gene in E. coli, you may use a. Topoisomerase I O b. Northern blotting O c. Primer extension Od. Foot-printing

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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To quantify the transcription product of a specific gene in E. coli, you may use
O a. Topoisomerase I
O b. Northern blotting
С.
Primer extension
O d. Foot-printing
Transcribed Image Text:To quantify the transcription product of a specific gene in E. coli, you may use O a. Topoisomerase I O b. Northern blotting С. Primer extension O d. Foot-printing
Expert Solution
Introduction

Proteins are a type of macromolecules that are critical for different varieties of roles in cellular functions. A protein is made up of 20 different amino acids combined in various ways.

Biosynthesis of proteins occurs in 2 steps:

  • Transcription - a process of producing mRNA from the gene.
  • Translation - a process of producing protein from the mRNA.

The three major types of RNA found in a cell include:

  • mRNA (Messenger RNA)
  • rRNA (Ribosomal RNA)
  • tRNA (Transfer RNA)
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