Create a hybrid cDNA-mRNA molecule using reverse transcriptase and oligo-dT primer, what is step 3 of making a cDNA library?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Create a hybrid cDNA-mRNA molecule using reverse transcriptase and oligo-dT primer, what is step 3 of making a cDNA library?

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Step 1

Deoxyribonucleic acid (DNA) is a genetic material containing thousands of genes which code for various amino acids and proteins for the functioning of the body. The DNA formed in the nucleus is condensed into chromosomes packed with histone proteins which help in keeping the strands of DNA intact and untangled. The double-helical structure of DNA and its structural properties was given by watson and crick. 

Ribonucleic acid (RNA) also contains genetic material but rarely takes part in transferring the genes from one generation to another like DNA. Instead, it plays a vital role in ribosome and protein synthesis. RNA forms in the nucleolus, and then moves to the cytoplasm. Types of RNA are, mRNA (messenger RNA), tRNA (transfer RNA) and rRNA (ribosomal RNA).

 

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