You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown on the right, what is the order of these four genes along the bacterial chromosome? Explain your reasoning.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
You co-culture the following bacterial strains: an Hfr prototroph and an F- auxotroph for the genes mal, met, mtl, and xyl. You interrupt conjugation at various time points and place the mixtures on media plates lacking each of the nutrients. Based on the results shown on the right, what is the order of these four genes along the bacterial chromosome? Explain your reasoning.
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