What is the difference between an F+ and an Hfr strain? Which type of strain do you expect to transfer many bacterial genes to recipient cells?
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
What is the difference between an F+ and an Hfr strain? Which type of strain do you expect to transfer many bacterial genes to recipient cells?
Bacterial conjugation is a type of gene transfer in which one bacterium transfers genetic material to another through direct contact. During conjugation, one bacterium serves as the donor of the genetic material, and the other serves as the recipient. It is a cell-cell contact dependent DNA transfer event.
The differences between F+ and Hfr strains are listed below:
|
F+ strain |
Hfr strain |
|
The fertility factor is independent of F+. |
The fertility factor is incorporated into the chromosomal DNA of the host cell. |
|
They can produce Hfr strain. |
They cannot produce F+ strain. |
|
They can conjugate only with F+ strain. |
They conjugate only with Hfr strain. |
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