Phage DNA- he Phage Infects bactertal coll Donor coll- (Infacted by a virus) that Is hes. Host DNA Is digostad Into fragmonts, and phago DNA and proteins are made. his Phagas assemble; occasionally a phago carrles a fragment of the host cell chromosome. In this casa, It carrles the hts gane Transduction in bacteria. Genes+Traits During transduction, a phage carries a segment of bacterial DNA from a donor to a recipient cell. In this case, the phage carried a segment of DNA with the his+ gene and transferred this gene to a recipient cell that was originally his- (unable to synthesize histidine). Following transduction, the recipient cell became his+, and thus able to synthesize histidine. Transducing phage with host DNA Transducing phage Injects Its DNA into a recipient cell that Racombination his- Raciplent coll- The transduced DNA Is recombined Into the chromosome of the reciplent cell. his Recombinant bacterium The racombinant bacterium's genotype has changed from his- to his".
Genetic Recombination
Recombination is crucial to this process because it allows genes to be reassorted into diverse combinations. Genetic recombination is the process of combining genetic components from two different origins into a single unit. In prokaryotes, genetic recombination takes place by the unilateral transfer of deoxyribonucleic acid. It includes transduction, transformation, and conjugation. The genetic exchange occurring between homologous deoxyribonucleic acid sequences (DNA) from two different sources is termed general recombination. For this to happen, an identical sequence of the two recombining molecules is required. The process of genetic exchange which occurs in eukaryotes during sexual reproduction such as meiosis is an example of this type of genetic recombination.
Microbial Genetics
Genes are the functional units of heredity. They transfer characteristic information from parents to the offspring.
In a P1 transduction experiment, the P1 lysate contains phages that
carry pieces of the host chromosomal DNA, but the lysate also contains
broken pieces of chromosomal DNA. If a P1 lysate is used to transfer chromosomal DNA to another bacterium, how could you show experimentally that the recombinant bacterium has been transduced (i.e., has taken up a P1 phage with a piece of chromosomal DNA inside) versus transformed (i.e., has taken up a piece of chromosomal DNA that is not within a P1 phage coat)?
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