14) Which antibiotic plasmid has been used? (kanamycin, ampicillin or tetracycline?) 15) Which of the following choices is best explanation for these results? (a, b, c or d?) a. Plates I and II each contain a plasmid that is resistant to that antibiotic. b. Plate II has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II

Q: A student wishes to transform some E. coli with a plasmid to “knock-out” (ie. “silence” or prevent)…

A: The transformation is the natural process in which the donor bacteria releases the free DNA in the…

Q: Looking at the model data i upload and given the question how could i analyse tboth experimental…

A: Agarose gel electrophoresis is the separation of DNA on the basis of size, supercoiled DNA will move…

Q: Using the plasmid map attached and the answer from the previous question, draw a simplified diagram…

A: The image you provided shows a diagram of the YCplac33 plasmid. This plasmid is a commonly used…

Q: The transfection reagent used to introduce plasmids to the HEK293 cells was a lipid reagent. Why is…

A: Transfection is the incorporation of DNA, RNA, or proteins in to the eukaryotic cells for the…

Q: What technique could be used to confirm the presence or absence of plasmids in the donor, recipient…

A: Microbiology is the branch of biology that deals with study of organisms that are too small to be…

Q: The ____ plasmid contains genes for synthesizing connections between donor and recipient cells.

A: The plasmid is defined as a small, circular, extra-chromosomal, double-stranded DNA molecule present…

Q: 4. The image below is based on the Meselson Stahl experiment where bacteria are grown in 15N and…

A: The Meselson-Stahl experiment showed that DNA replication is a semiconservative process. In this…

Q: A plasmid provides its host with kanamycin resistance. What will happen to the plasmid if you…

A: Plasmids are defined as extrachromosomal circular genetic elements that are capable of…

Q: Which plasmid contains the therapeutic gene? 1 - the plasmids with SERCA2a 2- the viral plasmid 3-…

A: The adenoviruses are generally used for making recombinant animal cells.

Q: Jm109 cells transformed with ligation and plasmid alone is used. The colonies most like:   white…

A: β-galactosidase is an enzyme encoded by the lacZ gene and is used to break down lactose to glucose…

Q: What is the purpose of arabinose in the pGLO lab, where is arabinose located, how can it be…

A: Arabinose acts as an allosteric regulator of AraC changing and tells which DNA sites it binds to and…

Q: How can you tell if you have had a successful induced transformation using: "C" = control, "T"…

A: Transformation refers to the process of introducing foreign DNA (such as plasmid DNA) into bacterial…

Q: Distinguish between a plate with individual colonies and a plate with a lawn. Under what conditions…

A: Introduction  A pure culture is a population of cells or multicellular organisms growing in the…

Q: What is the role of the following in the alkaline plasmid screen?    G buffer (Cell Suspension…

A: G buffer (Cell suspension solution): It is a solution that consists of a mixture of Tris (pH 7.5)…

Q: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)

A: Plasmids are the extrachromosomal material that exist apart from the main chromosome in a bacterial…

Q: Prophages and proviruses have been described as being similar to bacterial plasmids. How are…

A: Several molecular structures and techniques are used in genetics for the manipulation of genes or to…

Q: Many studies have linked plasmids to bacterial resistance to antibiotics.  Closured circular…

A: Focusing on plasmids as a target to combat antibiotic resistance holds promise but requires a…

Q: Why should plasmid sample be mixed in the inverted position and not vortexed?  Will these actions…

A: Plasmids are small circular pieces of DNA that can be found in many bacteria and are often used in…

Q: EDTA weakens the cell wall by removing ions that help hold it together while glucose prevents…

A: EDTA disrupts the outer membrane (enzymes that degrade DNA) by inhibiting DNases and chelating…

Q: Each of the plates was inoculated with E. coli strain 1 that carry a plasmid with Str resistance and…

A: Introduction  A plasmid is a small, circular, double-stranded DNA molecule that exists outside the…

Q: 16. Which of the following methods may introduce foreign DNA into a recipient? A) transduction B)…

A: A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's…

Q: where are the bacteria that did not take up the plasmid during the transformation

A: Introduction : The technique of horizontal gene transfer known as bacterial transformation allows…

Q: You are studying a group of individuals with X-ray vision and perform linkage analysis and note that…

A: DNA cloning is the procedure in genetic engineering used for the production of a larger number of…

Q: a. What is the function of the SDS in the lysis buffer? the NaOH? b. What does the potassium…

A: These question are regarding plasmid DNA isolation

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.