4 I.I 5 | I6 IUse the following information to answer questions 14-15.A student has forgotten which antibiotic plasmid she used in her E. coli transformation. It could have beenkanamycin, ampicillin, or tetracycline. She decides to make up a special set of plates to determine the typeof antibiotic used. The plates below show the results of the test.Plate I. LB agarwith kanamycinPlate II. LB agarwith ampicillinPlate III. LB agarwith tetracyclinePlate IV. LB agar14) Which antibiotic plasmid has been used?(kanamycin, ampicillin or tetracycline?)(a, b, c or d?)15) Which of the following choices is best explanation for these results? 14) Which antibiotic plasmid has been used?(kanamycin, ampicillin or tetracycline?)15) Which of the following choices is best explanation for these results?(a, b, c or d?)a. Plates I and II each contain a plasmid that is resistant to that antibiotic.b. Plate III has antibiotic agar, but E. coli that has been transformed to be resistant totetracycline can grow.c. Plate IV has no antibiotic.d. There are no tetracycline-resistant cells on Plate II

14 ) tetracycline is the antibiotic plamid.

14) Which antibiotic plasmid has been used? (kanamycin, ampicillin or tetracycline?) 15) Which of the following choices is best explanation for these results? (a, b, c or d?) a. Plates I and II each contain a plasmid that is resistant to that antibiotic. b. Plate II has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II

14) Which antibiotic plasmid has been used? (kanamycin, ampicillin or tetracycline?) 15) Which of the following choices is best explanation for these results? (a, b, c or d?) a. Plates I and II each contain a plasmid that is resistant to that antibiotic. b. Plate II has antibiotic agar, but E. coli that has been transformed to be resistant to tetracycline can grow. c. Plate IV has no antibiotic. d. There are no tetracycline-resistant cells on Plate II

Biology: The Unity and Diversity of Life (MindTap Course List)

14th Edition

ISBN:9781305073951

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 12SQ: Which of the following can be used to carry foreign DNA into host cells? Choose all correct answers....

See similar textbooks

Similar questions

B ut of 1 e flag us page In Canada, facilities that participate in sterile compounding follow guidelines provided by USP Chapter , NAPRA and this professional association: Select one: O a. CAPT Q b. CPTEA O C. FDA O d. CSHP FS Q Search F6 - F7 F8 L F9 m |--- ■■■ 8 + Time left 21:19: F10 Next page
Which of the following can be used to carry foreign DNA into host cells? Choose all correct answers. a. RNA b. viruses c. PCR d. plasmids e. lipid clusters f. blasts of pellets g. xenotransplantation h. nanoparticles
List the antibiotics that could successfully be used to test for the presence of the 4. gene on your plasmid.
explain the steps for restriction and how each procedure of these three routes is performed: 1.SQ 2.IM 3.IV
1. You take a sample with a concentration of 1,000,000 pfu/mL of bacteriophage and make a dilution by taking 1mL from the tube and add it to 9mL of fresh broth and mix. You and repeat this 4 more times, Then you plate 1mL of the sample with a few drops of E.coli on a TSA plate. How many plaques do you expect to see when you examine the plate the next day? m^^^? Tube A a) 1,000,000 plaques b) 1000 plaques c) 10 plaques d) 1 plaque e) No plaques E. coli TSA
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in.
PART C (cont'd) Which of the following steps (on this page and the next page) would be necessary to determine the most effective antibiotic for a bacterial infection using the Disk Diffusion Assay? Select three necessary steps and three unnecessary or incorrect steps, and explain why it is or is not necessarv/correct. Collect a sample of bacteria from the infected patient. Collect samples of bacteria from the bathroom taps in the patient's room. Collect samples of bacteria from other patients who seem to have the same infection. Soak disks in many different types of antibiotic. Soak disks in the antibiotic you have the biggest supply ot. Label the disks with the type of antibiotic you soaked them in. • Leave the petri dishes uncovered while the bacteria grow. , cover the petri dishes while the bacteria grow. Treat the bacterial culture with a disinfectant before spreading on the agar plate Wear personal protective equipment (gloves, mask, lab coat) while performing the procedure Wear…
im a bit confused on the answer I was given for question 4, I believe the question is asking to discuss how genomic analysis would inform therapeutic choices, I am specifically looking for some therapy choices for malignant melanoma and to link them to the genomic analysis given
Row C D. B B. one: A C O phage with radiolabelled protein coat phage with radiolabelled DNA 100 10. phage infects The experiment shown above was designed by bacterium phage infects bacterium EXPERIMENT 1 phage shell is removed EXPERIMENT 2 요 Hershey & Hershey & Chase Meselson & Stahl Meselson & Stahl 8 phage shell is removed 28 no radioactivity in cells ii 48 radioactivity in cells LL and proved that ii DNA replication is semiconservative DNA is the hereditary material DNA replication is semiconservative DNA is the hereditary material (select the row that correctly completes the statement)
Write the letter of your choice at the start of the sentence then support it with a short explanation. Plaque assay and Kirby Bauer assay are similar in terms of A. they both use a bacterial lawn B. zone of inhibition is measured C. antibiotics are used D. agar overlay is added on solidified agar
Define/Compare/Contrast/Distinguish these three terms: F plasmid F’ plasmid (and how is it formed) Hfr cell (and how is it formed)
Pipetting 1. Explain why a micropipettor is an important instrument in biochemistry labs. 2. Describe the basic components (and function) of a micropipettor. Bacterial Techniques 1. Define the following. (a) Serial Dilution (b) Streak Plating (c) Spread Plating Transformation 1. Define bacterial transformation and explain why it is an important method in biochemistry labs. 2. Describe (figure, narrative) a plasmid and describe the basic components of a plasmid. 3. What role does CaCl2 play in bacterial transformation? 4. What role does heat shock play in bacterial transformation? Plasmid Isolation 1. Describe the roles the following play in plasmid purification. (a) Lysis Buffer (b) Neutralization Buffer (c) Elution Buffer