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A cell line carrying a mutation in a single gene is found to express very low levels of MHC class I on its surface. When infected with influenza virus, these cells are not recognized nor are they killed by a CD8 T cell line specific for an influenza peptide bound to the MHC class I protein expressed by these cells. Incubation of the mutant cell line with a large excess of this peptide in the cell culture medium overnight leads to the results shown below.  What is the most likely candidate for the gene that is defective in the mutant cell line?

A cell line carrying a mutation in a single gene is found to express very low levels of MHC class I on its surface. When infected with influenza virus, these cells are not recognized nor are they killed by a CD8 T cell line specific for an influenza peptide bound to the MHC class I protein expressed by these cells. Incubation of the mutant cell line with a large excess of this peptide in the cell culture medium overnight leads to the results shown below.  What is the most likely candidate for the gene that is defective in the mutant cell line?

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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A cell line carrying a mutation in a single gene is found to express very low levels of MHC class I on its surface. When infected with influenza virus, these cells are not recognized nor are they killed by a CD8 T cell line specific for an influenza peptide bound to the MHC class I protein expressed by these cells. Incubation of the mutant cell line with a large excess of this peptide in the cell culture medium overnight leads to the results shown below.  What is the most likely candidate for the gene that is defective in the mutant cell line?

### Wild Type vs. Mutant MHC Class I Expression This image contains a comparative analysis of MHC Class I expression in wild type and mutant cells, both in the presence and absence of peptides. #### Without Peptide (- peptide) - **Wild Type**: - The graph on the left side displays two overlapping histograms for MHC Class I expression. - The grey histogram represents a control or baseline expression level. - The red histogram signifies the level of expression specific to the wild type without peptide. - **Mutant**: - On the right, the mutant cell's MHC Class I expression is depicted. - The grey and blue histograms represent the control level and the mutant MHC Class I expression, respectively. - The mutant MHC Class I is slightly shifted compared to the control, indicating a different expression pattern compared to the wild type. #### With Peptide (+ peptide) - **Wild Type**: - The graph shows the same format: grey for the control and red for the wild type with peptide. - Compared to the left graph without peptide, there is a noticeable increase in the MHC Class I expression levels (as indicated by the increase in red area). - **Mutant**: - The grey histogram remains the control level. - The blue histogram shows the MHC Class I expression in the mutant with peptide. - There is an increase compared to the mutant without peptide, signifying that the addition of peptide enhances MHC Class I expression but with a distinct distribution compared to the wild type. ### Interpretation - In the absence of peptide, both the wild type and mutant express MHC Class I, but the levels are noticeably different. - The presence of peptide significantly enhances MHC Class I expression in both cell types, suggesting that peptide stimulation is crucial for optimal MHC Class I presentation. - Wild type cells show a more pronounced shift in histogram distribution upon peptide addition compared to mutant cells. This diagram highlights the differential expression patterns between wild type and mutant MHC Class I, demonstrating the significant role peptides play in MHC Class I expression.
Transcribed Image Text:### Wild Type vs. Mutant MHC Class I Expression This image contains a comparative analysis of MHC Class I expression in wild type and mutant cells, both in the presence and absence of peptides. #### Without Peptide (- peptide) - **Wild Type**: - The graph on the left side displays two overlapping histograms for MHC Class I expression. - The grey histogram represents a control or baseline expression level. - The red histogram signifies the level of expression specific to the wild type without peptide. - **Mutant**: - On the right, the mutant cell's MHC Class I expression is depicted. - The grey and blue histograms represent the control level and the mutant MHC Class I expression, respectively. - The mutant MHC Class I is slightly shifted compared to the control, indicating a different expression pattern compared to the wild type. #### With Peptide (+ peptide) - **Wild Type**: - The graph shows the same format: grey for the control and red for the wild type with peptide. - Compared to the left graph without peptide, there is a noticeable increase in the MHC Class I expression levels (as indicated by the increase in red area). - **Mutant**: - The grey histogram remains the control level. - The blue histogram shows the MHC Class I expression in the mutant with peptide. - There is an increase compared to the mutant without peptide, signifying that the addition of peptide enhances MHC Class I expression but with a distinct distribution compared to the wild type. ### Interpretation - In the absence of peptide, both the wild type and mutant express MHC Class I, but the levels are noticeably different. - The presence of peptide significantly enhances MHC Class I expression in both cell types, suggesting that peptide stimulation is crucial for optimal MHC Class I presentation. - Wild type cells show a more pronounced shift in histogram distribution upon peptide addition compared to mutant cells. This diagram highlights the differential expression patterns between wild type and mutant MHC Class I, demonstrating the significant role peptides play in MHC Class I expression.
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