Answered: The diosgenin drug (DSG) has been…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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The diosgenin drug (DSG) has been tested on human cholangiocarcinoma cells (bile duct cancer cells). To this aim, the human cholangiocarcinoma cell lines HuCCT1, QBC939, SK-ChA-1, HuH28, RBE, and Mz-ChA-1 have been subjected to different conditions of treatment with DSG, then assessed for their viability using a MTS assay, and their cell cycle progression using flow cytometry (FCM).
Note: The MTS assay is based on the reduction of the MTS tetrazolium compound by viable cells to generate a colored formazan dye that is soluble in cell culture media. This conversion is thought
to be carried out by NAD(P)H-dependent dehydrogenase enzymes in metabolically active cells.
The formazan dye is quantified by measuring its absorbance at 490-500 nm.

Question : To which kind of biological molecule does this DSG drug resemble? To which kind of properties do you expect for DSG?

Figure 2 presents the semi-developed formula of the DSG drug.
CH3
H;C
H3C
Figure 2: semi-developed formula of
the DSG drug
H3C
H.
H,
HO

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