You’re researching the effect of two drugs (Drug A and Drug B) on colon carcinoma cells in vitro. You begin by performing an assay to determine the effect of the drugs on cell viability and generate the data below in the graph below (A). With the aim of determining the mechanism of action of the drugs, you subsequently investigate levels of several key apoptotic proteins via SDS-PAGE and Western blot (B). To measure cytochrome C, you first separate the mitochondrial fragments from the cytosolic fragments and measure the levels of cytochrome C in each. You measure the remaining proteins using whole-cell lysates. B-Actin was included as a loading control. This data is represented on the right below. Part 1 Which drug is most likely inducing apoptosis? Explain your reasoning with referent to the cellular events that have taken place in response to the drugs, based on the data Part 2 Is it possible to determine from this data I the activation of apoptosis is occurring through the intrinsic pathway? Why or why not? What else could be measured in this experiment to confirm this? Part 3 Both Drugs A& B reduce the population of viable cells. How else could this cell death occur if not by apoptosis, and would this drugs capacity as a therapeutic?
You’re researching the effect of two drugs (Drug A and Drug B) on colon carcinoma cells in vitro. You begin by performing an assay to determine the effect of the drugs on cell viability and generate the data below in the graph below (A). With the aim of determining the mechanism of action of the drugs, you subsequently investigate levels of several key apoptotic proteins via SDS-PAGE and Western blot (B). To measure cytochrome C, you first separate the mitochondrial fragments from the cytosolic fragments and measure the levels of cytochrome C in each. You measure the remaining proteins using whole-cell lysates. B-Actin was included as a loading control. This data is represented on the right below. Part 1 Which drug is most likely inducing apoptosis? Explain your reasoning with referent to the cellular events that have taken place in response to the drugs, based on the data Part 2 Is it possible to determine from this data I the activation of apoptosis is occurring through the intrinsic pathway? Why or why not? What else could be measured in this experiment to confirm this? Part 3 Both Drugs A& B reduce the population of viable cells. How else could this cell death occur if not by apoptosis, and would this drugs capacity as a therapeutic?
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