Jurkat cells are an immortalized line of human T-lymphocytes cells that are used to studyacute T cell leukemia. K562 cells were the first human immortalized myelogenousleukemia cell line to be established. Resting= control, Etoposide= drug. Below is shownthe results of floweytometry of untreated and treated cells. c. Would a drug that induced apoptosis or one that induced necrosis be preferable fortreatment of cancer? Why?d. Why might there be different results for Etoposide as an anti-cancer agent fordifferent cell lines?A) Resting JurkatcellsB) Jurkatcells- Etoposide10.29so DeadDeadLiveAPOLiveAPO57.110a 101010Annoxin VFiTcAAnnexn V FITCAC) Resting K562 cells14,86DeadD) K562 cells-EtoposideDead335114LiveAPOLiveAPOAnnexin V incA10Annexin VnCALegend. Jurkat & K562 cells were incubated with 10µM etoposide for 24h. RestingJurkat cells A), etoposide treated Jurkat cells B), resting K662 cells C) etoposidetreated K562 celle D) were labeled with annexin V-FITC and DAPI (200ng/mi).Courtesy of the Flow Cytometry Core Facility, Blizard institute, Barts & The LondonSchool of Medicine & Dentistry, Queen Mary University of London, E1 2AT.

INTRODUCTION Flowcytometry Flowcytometry is a technique that simultaneously measures and analyse…

Would a drug that induced apoptosis or one that induced necrosis be preferable for treatment of cancer? Why? Why might there be different results for Etoposide as an anti-cancer agent for different cell lines?

Would a drug that induced apoptosis or one that induced necrosis be preferable for treatment of cancer? Why? Why might there be different results for Etoposide as an anti-cancer agent for different cell lines?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter12: Genes And Cancer

Section: Chapter Questions

Problem 20QP: Can you postulate a reason or reasons why children with Down syndrome are 20 times more likely to...

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Concept explainers

Genomic studies

Genomic studies (genomics) is the study of the whole genome of an organism. Using bioinformatics and high-performance computing, genomic researchers examined massive amounts of deoxyribonucleic acid (DNA)-sequence data to discover variations or mutations that affect drug response, health, or disease. Genomics is a much younger discipline than genetics, having only emerged in the last few decades as a result of technological advancements in DNA sequencing and computational biology.

Question

Jurkat cells are an immortalized line of human T-lymphocytes cells that are used to study
acute T cell leukemia. K562 cells were the first human immortalized myelogenous
leukemia cell line to be established. Resting= control, Etoposide= drug. Below is shown
the results of floweytometry of untreated and treated cells.

c. Would a drug that induced apoptosis or one that induced necrosis be preferable for
treatment of cancer? Why?
d. Why might there be different results for Etoposide as an anti-cancer agent for
different cell lines?
A) Resting Jurkatcells
B) Jurkatcells- Etoposide
10.29
so Dead
Dead
Live
APO
Live
APO
57.1
10
a 10
10
10
Annoxin VFiTcA
Annexn V FITCA
C) Resting K562 cells
14,86
Dead
D) K562 cells-Etoposide
Dead
335
114
Live
APO
Live
APO
Annexin V incA
10
Annexin VnCA
Legend. Jurkat & K562 cells were incubated with 10µM etoposide for 24h. Resting
Jurkat cells A), etoposide treated Jurkat cells B), resting K662 cells C) etoposide
treated K562 celle D) were labeled with annexin V-FITC and DAPI (200ng/mi).
Courtesy of the Flow Cytometry Core Facility, Blizard institute, Barts & The London
School of Medicine & Dentistry, Queen Mary University of London, E1 2AT.

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