graduate student wants to isolate cells from a patient and grow them perpetually in culture to study a non-cancer related pathology. Which two of the following genes could you introduce into these cells under control of a constitutive promoter to propogate these cells continuously in culture (select two)? A. Telomerase B. Retinoblastoma C. MDM2 D. p21 CIP E. SV40 Large T Antigen
The correct answers are as follows-
A. Telomerase
C. MDM2
A graduate student wants to isolate cells from a patient and grow them perpetually in culture to study a non-cancer-related pathology. The genes that encode telomerase and MDM2 should be introduced into these cells under the control of a constitutive promoter to propagate these cells continuously in culture. When the promoter is constitutively active, it will be active all the time, irrespective of the silencing cues. The cell line will become immortal when the telomerase and MDM2 are always active. Telomerase is the enzyme that adds telomeric sequence at the ends of the chromosomes and prevents ageing. MDM2 is the negative regulator of the tumour suppressor p53, activated during checkpoint-related problems of the cell cycle. So, both genes should always be active in the cell to propagate them.
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