PYK gene codes for the expression of pyruvate kinase, which is one of the enzymes targeted for anti-cancer drug design. You have identified an RNAi that targets the mRNA of PYK gene. To study the effect of the RNAi towards pyruvate kinase, the respected

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
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Chapter1: The Human Body: An Orientation
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Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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The PYK gene codes for the expression of pyruvate kinase, which is one of the enzymes
targeted for anti-cancer drug design. You have identified an RNAi that targets the mRNA
of PYK gene. To study the effect of the RNAi towards pyruvate kinase, the respected RNAi
is expressed in Saccharomyces cerevisiae. The level of pyruvate kinase can be detected
with a fluorescent antibody.
(a). Predict the result that you will obtain in recombinant S. cerevisiae that expresses the
respected RNAi.
(b). Compare the result in Q3a(i) with the wild-type S. cerevisiae.

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