Recall that the trp operon has a special leader sequence (trpL) between the operator and the structural genes that offers attenuation as a mechanism for regulation of gene expression. (A) Draw a diagram of a trpL region of the operon when tryptophan is abundant in the cell. Label the following features: the DNA, 5’ and 3’ polarity of the RNA, the regions 1, 2, 3, and 4 and poly-U of the RNA, the pair of Trp codons (UGG), the ribosome, and RNA-Pol, along with any stem-loop structure that would form under these conditions (B) In the above example, will the rest of the trp operon genes be expressed? Briefly describe your reasoning why or why not (C) The trp codons in region 1 of the trpL gene have mutated to cysteines (UGG to UGC). What will be the effect on attenuation gene regulation of the trp operon? Brieflyexplain your reasoning.
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. Recall that the trp operon has a special leader sequence (trpL) between the operator and the structural genes that offers attenuation as a mechanism for regulation of gene expression.
(A) Draw a diagram of a trpL region of the operon when tryptophan is abundant in the cell.
Label the following features: the DNA, 5’ and 3’ polarity of the RNA, the regions 1, 2, 3,
and 4 and poly-U of the RNA, the pair of Trp codons (UGG), the ribosome, and RNA-Pol,
along with any stem-loop structure that would form under these conditions
(B) In the above example, will the rest of the trp operon genes be expressed? Briefly describe your reasoning why or why not
(C) The trp codons in region 1 of the trpL gene have mutated to cysteines (UGG to UGC). What will be the effect on attenuation gene regulation of the trp operon? Brieflyexplain your reasoning.
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