(a) Did deletion of any of the possible control elements cause anincrease in reporter gene expression relative to the control? If so,which one(s), and how can you tell? (b) If loss of a control elementcauses an increase in gene expression, what must be the normalrole of that control element? Propose a biological explanation forhow the loss of such a control element could lead to an increase ingene expression.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
(a) Did deletion of any of the possible control elements cause an
increase in reporter gene expression relative to the control? If so,
which one(s), and how can you tell? (b) If loss of a control element
causes an increase in gene expression, what must be the normal
role of that control element? Propose a biological explanation for
how the loss of such a control element could lead to an increase in
gene expression.
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