Table 1: Preparation of Ascorbic Acid Standards Tube Concentration of Volume Ascorbic Acid Ascorbic acid Volume of DI H2O Total Volume (uM) (10 mM stock solution) 1 0 uM 0 uL 1000 uL 1 mL 2 50 uM 5 UL 995 uL 1 mL 3 100 uM 10 uL 990 uL 1 mL st 4 150 uM 15 uL 985 uL 1 mL 5 LO 200 uM 20 uL 980 uL 1 mL CO 6 300 uM 30 uL 970 uL 1 mL
Table 1: Preparation of Ascorbic Acid Standards Tube Concentration of Volume Ascorbic Acid Ascorbic acid Volume of DI H2O Total Volume (uM) (10 mM stock solution) 1 0 uM 0 uL 1000 uL 1 mL 2 50 uM 5 UL 995 uL 1 mL 3 100 uM 10 uL 990 uL 1 mL st 4 150 uM 15 uL 985 uL 1 mL 5 LO 200 uM 20 uL 980 uL 1 mL CO 6 300 uM 30 uL 970 uL 1 mL
Chapter9: Parenteral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 4.8P
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I am a Biochemistry student and I am confused on how to analyze FRAP Analysis using Excel Spread Sheets.
The following spread sheet has my 0 minute data listed at top and the 4 minute data listed on the bottom.
Sheet: https://mnscu-my.sharepoint.com/:x:/g/personal/vi2163ss_go_minnstate_edu/EXjrCizWiXRPmpittqZA12IB8EkB5eE8iaRqj_iun-IAtg?rtime=Wo9zPHFY3Ug
The formula for FRAP Analysis is:
FRAP value = A (4 min sample) - A (0 min sample) over A (4 min 30 uM ascorbic acid) - A (0 min 30 uM ascorbic acid) multiplied by 30 uM and the dilution factor of 1/10
Transcribed Image Text:Table 1: Preparation of Ascorbic Acid Standards
Tube
Concentration of
Volume
Ascorbic Acid
Ascorbic acid
Volume of DI
H2O
Total Volume
(uM)
(10 mM stock
solution)
1
0 uM
0 uL
1000 uL
1 mL
2
50 uM
5 UL
995 uL
1 mL
3
100 uM
10 uL
990 uL
1 mL
st
4
150 uM
15 uL
985 uL
1 mL
5
LO
200 uM
20 uL
980 uL
1 mL
CO
6
300 uM
30 uL
970 uL
1 mL
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