Lab 1 Enzyme Kinetics 4. Use the molecular weight of tyrosinase, 128,000, to determine how many micromoles of tyrosinase are in the tubes that you used to calculate Va Enzyme Volume 0.500 ml or 0.600 ml MW-128,000 g/mol→ 128,000 mg/mmol Stock concentration-0.2 mg/ml* 0.500 ml [E]= ?μmol 1 mmol 128,000 mg 0.2 mg 0.5 ml 5. The turnover number is the number of moles of product produced per minutes by 1 mol of enzyme at V. Using the number you calculated in item 4 and the Vmax from your linear graph, calculate the turnover number for tyrosinase.

Lab 1 Enzyme Kinetics 4. Use the molecular weight of tyrosinase, 128,000, to determine how many micromoles of tyrosinase are in the tubes that you used to calculate Va Enzyme Volume 0.500 ml or 0.600 ml MW-128,000 g/mol→ 128,000 mg/mmol Stock concentration-0.2 mg/ml* 0.500 ml [E]= ?μmol 1 mmol 128,000 mg 0.2 mg 0.5 ml 5. The turnover number is the number of moles of product produced per minutes by 1 mol of enzyme at V. Using the number you calculated in item 4 and the Vmax from your linear graph, calculate the turnover number for tyrosinase.

Biochemistry

9th Edition

ISBN:9781305961135

Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Chapter6: The Behavior Of Proteins: Enzymes

Section: Chapter Questions

Problem 31RE: MATHEMATICAL You do an enzyme kinetic experiment and calculate a Vmax of 100 mol of product per...

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