The amino acid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine, which has a pKa of 9.6, can exist either in the protonated form -NH or as the free base -NH2, because of the reversible equilibrium R-NH =R-NH₂ + H+ In what pH range can glycine be used as an effective buffer due to its amino group? pH 8.6 to pH 10.6 In a 0.1 M solution of glycine at pH 9.0, what fraction of glycine has its amino group in the -NH form? Correct Answer Correct Answer 45 How much 5 M KOH must be added to 1.0 L of 0.1 M glycine at pH 9.0 to bring its pH to 10.0? 10 mL When 99% of the glycine is in its -NH form, what is the numerical relation between the pH of the solution and the pKa of the amino group? pH = pKa - 2 Correct Answer Correct Answer
The amino acid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine, which has a pKa of 9.6, can exist either in the protonated form -NH or as the free base -NH2, because of the reversible equilibrium R-NH =R-NH₂ + H+ In what pH range can glycine be used as an effective buffer due to its amino group? pH 8.6 to pH 10.6 In a 0.1 M solution of glycine at pH 9.0, what fraction of glycine has its amino group in the -NH form? Correct Answer Correct Answer 45 How much 5 M KOH must be added to 1.0 L of 0.1 M glycine at pH 9.0 to bring its pH to 10.0? 10 mL When 99% of the glycine is in its -NH form, what is the numerical relation between the pH of the solution and the pKa of the amino group? pH = pKa - 2 Correct Answer Correct Answer
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Transcribed Image Text:The amino acid glycine is often used as the main ingredient of a buffer in biochemical experiments. The amino group of glycine,
which has a pKa of 9.6, can exist either in the protonated form -NH or as the free base -NH2, because of the reversible
equilibrium
R-NH =R-NH₂ + H+
In what pH range can glycine be used as an effective
buffer due to its amino group?
pH 8.6 to pH 10.6
In a 0.1 M solution of glycine at pH 9.0, what
fraction of glycine has its amino group in the
-NH form?
Correct Answer
Correct Answer
45
How much 5 M KOH must be added to 1.0 L of
0.1 M glycine at pH 9.0 to bring its pH to 10.0?
10 mL
When 99% of the glycine is in its -NH form, what
is the numerical relation between the pH of the
solution and the pKa of the amino group?
pH = pKa - 2
Correct Answer
Correct Answer
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