In a radiolabeled ligand assay to determine the binding affinity for an agonist, different concentrations of agonist are placed in test tubes with both a set number of receptors and radiolabeled ligand. Filters separate the bound and free radioligand, and a scintillation counter measures the number of bound radioligand. But how is the binding affinity of the agonist determined from all this?

In a radiolabeled ligand assay to determine the binding affinity for an agonist, different concentrations of agonist are placed in test tubes with both a set number of receptors and radiolabeled ligand. Filters separate the bound and free radioligand, and a scintillation counter measures the number of bound radioligand. But how is the binding affinity of the agonist determined from all this?

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.7: Bacterial Identification And Antibiotic Susceptibility Testing

Problem 11RQ

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