In a radiolabeled ligand assay to determine the binding affinity for an agonist, different concentrations of agonist are placed in test tubes with both a set number of receptors and radiolabeled ligand. Filters separate the bound and free radioligand, and a scintillation counter measures the number of bound radioligand. But how is the binding affinity of the agonist determined from all this?
In a radiolabeled ligand assay to determine the binding affinity for an agonist, different concentrations of agonist are placed in test tubes with both a set number of receptors and radiolabeled ligand. Filters separate the bound and free radioligand, and a scintillation counter measures the number of bound radioligand. But how is the binding affinity of the agonist determined from all this?
Principles Of Radiographic Imaging: An Art And A Science
6th Edition
ISBN:9781337711067
Author:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Publisher:Richard R. Carlton, Arlene M. Adler, Vesna Balac
Chapter42: Diagnostic Medical Sonography
Section: Chapter Questions
Problem 9RQ
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In a radiolabeled ligand assay to determine the binding affinity for an agonist, different concentrations of agonist are placed in test tubes with both a set number of receptors and radiolabeled ligand. Filters separate the bound and free radioligand, and a scintillation counter measures the number of bound radioligand. But how is the binding affinity of the agonist determined from all this?
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