Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?

5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
See similar textbooks
icon
Related questions
Question

Please help me with 5 d) 

5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?
Transcribed Image Text:5) Under specific conditions, hemoglobin dissociates into its four subunits. The isolated a subunit binds oxygen, but the O2-saturation curve is hyperbolic rather than sigmoidal. In addition, the binding of oxygen to the isolated a subunit is not affected by pH, or the allosteric regulator BPG. Use your knowledge about hemoglobin and the information provided in this question to answer: a. Draw the expected O₂ binding curves for tetrameric hemoglobin and for the isolated a subunit (draw them on the same axes). How can you explain their different behavior? b. Draw the expected Hill plot for tetrameric hemoglobin and for the isolated a subunit. c. What Hill coefficient (n) will you expect for tetrameric hemoglobin and for the isolated a subunit? d. How would you use each of these graphs to determine dissociation constants for the binding of oxygen to tetrameric hemoglobin and to the isolated a subunit? e. Why is the isolated a subunit unaffected by pH or BPG?
Expert Solution
steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Similar questions
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON
SEE MORE TEXTBOOKS