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You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.

You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Using this information solve for enzyme activity over time 1 unit - 1 umole per minute.Total reaction volume is 1ml Note: reaction was run for 15 minutes (use relavent formulas) 
please show full working out. The first part of this question is done 1:5 concentration = 144.7uM and 1:10 concentration 142.4uM. (Use this to solve for enzyme activity) 
refer to image for all other details and show all working out. Thanks 

You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.
Transcribed Image Text:You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.
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