You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.

You repeated the experiment and generated new fluorescence values for the samples and standards, shown below in the table. Standard Averaged fluorescence values 10 μΜ 20 ΜΜ 30 μM 40 μΜ 50 μΜ 0.121 0.326 0.586 0.728 1.105 Viral protease enzyme diluted 1:5 0.548 Viral protease enzyme diluted 1:10 0.200 Generate a standard curve. a) What is the equation of the best fit line of your standard curve? Note: Your answers need to be to four (4) decimal places. y = 0.0237 X- 0.1378 Calculate the viral protease enzyme activity in units based on the standard curve, if the reaction was run for 15 minutes. Hint: Calculate the concentration of the viral protease enzyme first, based on the equation of the standard curve and taking into account the dilution factors, then calculate the enzyme activity over time. 1 Unit = 1 μmole per minute Note: The total reaction volume is 1 ml. You must show your calculations to receive full marks.

Aquaculture Science

3rd Edition

ISBN:9781133558347

Author:Parker

Publisher:Parker

Chapter2: Aquatic Plants And Animals

Section: Chapter Questions

Problem 2KA

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