(d) From your plot, calculate the activity of the enzyme.Use the space below to show your workings:11k and all other necess PAEnzyme(a) Earlier, the activity for this protein/enzyme was tested by monitoring the concentration of theproduct A' as a function of time. The results are shown in the table below. Copy thesenumbers into an excel spreadsheet and prepare an enzyme activity plot. Be sure to add all thenecessary labels to your plot. (You don't need to copy the plot into this worksheet).Time (min)01234567A'89101112A' (μmol/mL)0.011.022.133.144.155.166.277.288.292.494.596.696.6(b) Which part of the data (in minutes) is suitable to use to calculate the activity of the Enzyme,and why? Write downs your answer in the space below.

Enzyme kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction rate…

Answered: (d) From your plot, calculate the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Similar questions

Using the table below can you please help me answer the following. Based on your results, what can you conclude about the effect of enzyme concentration on reaction rate? Explain
A post doc was working on charaterizing a new enzyme and found that in a 357 amino acid enzyme, the amino acids 33,34,35 and amino acids 301,303,306 form one active site. Q1:As the PI, would you tell him 1) that this result doesn't make any sense or 2) that this is a interesting finding? Explain your answer Q2: if the amino acid residues 33,34,35 were Phe,Val, and Ala, would this be possible to occur?
Draw the Michaelis-Menten graph you would predict for the enzyme using 50 nM and 100 nM of enzyme in your assays.
Consider the following free energy diagram for an uncatalyzed and enzyme-catalyzed reaction. Select all the statements that are true. Without enzyme With enzyme A+B Time AB Oa. The reaction is now spontaneous due to the addition of enzyme b. The rate of the enzyme catalyzed reaction is faster than the uncatalyzed reaction O C. The reaction is exergonic O d. The change in free energy for the reaction is greater in the catalyzed reaction, compared to the uncatalyzed reaction e. The enzyme stabilizes the transition state for the reaction Released Energy pes
Is the data that you are collecting in the above table quantitative or qualitative? Explain why. Which treatment had the least amount of browning? Which had the most? Why do you think you obtained these results? Remember that the enzyme polyphenol oxidase is a protein! For each treatment, apply your knowledge of how temperature, pH, and salt concentration affect enzyme activity and explain why you got the results that you did. Include bonds and the levels of protein structure that you explored in Activity A in your answer. How does temperature impact the rate of enzyme activity? If you were to leave the apple in the refrigerator longer, why would it eventually brown? Explain based on what you know about enzyme activity. How does pH and its impact on specific types of bonds explain the results you obtained in your lemon juice treatment? Include bonds and levels of protein structure in your answer. How does salt and its impact on specific types of bonds explain the results you obtained…
If 10 µM enzyme was used to obtain the data in the summary plot below, k-cat = ________________ sec-1 and efficiency = _________________ sec-1 M-1 for this enzyme. (Enter numeric answers to the nearest integer; Do NOT write unts.)

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(d) From your plot, calculate the activity of the enzyme. Use the space below to show your workings: