You are studying an enzyme and wish to determine how an inhibitor functions. The enzyme reaction is A->P. You perform two experiments, one measuring enzyme activity without inhibitor and one measuring enzyme activity with 200 uM inhibitor. The results are presented below: No inhibitor a (uM) v (uM/sec) 10 15 25 40 70 8.333 10.714 13.889 16.667 19.444 200 uM inhibitor a (UM) v (uM/sec) 10 15 25 40 70 6.250 7.500 8.929 10.000 10.938 What are Vmax, KM, K₁. What type of inhibition does I cause?
You are studying an enzyme and wish to determine how an inhibitor functions. The enzyme reaction is A->P. You perform two experiments, one measuring enzyme activity without inhibitor and one measuring enzyme activity with 200 uM inhibitor. The results are presented below: No inhibitor a (uM) v (uM/sec) 10 15 25 40 70 8.333 10.714 13.889 16.667 19.444 200 uM inhibitor a (UM) v (uM/sec) 10 15 25 40 70 6.250 7.500 8.929 10.000 10.938 What are Vmax, KM, K₁. What type of inhibition does I cause?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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You perform two experiments, one measuring enzyme activity without inhibitor and one measuring enzyme activity with 200 uM inhibitor. The results are presented below:
What are Vmax, KM, KI?
What type of inhibition does I cause?
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