A. Estimate from the graph what the Vmax is for the enzyme without inhibitor present (black circles) and in the presence of the inhibitor (green squares). B. Estimate the Km from the graph without inhibitor present (black circles) and in the presence of the inhibitor (green squares). C. Based on the data, what type of reversible inhibitor do you think was used? Explain your answer.
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
1.
A. Estimate from the graph what the Vmax is for the enzyme without inhibitor present (black circles) and in the presence of the inhibitor (green squares).
B. Estimate the Km from the graph without inhibitor present (black circles) and in the presence of the inhibitor (green squares).
C. Based on the data, what type of reversible inhibitor do you think was used? Explain your answer.
![Part D: Measuring the impact of substrate concentration
Michaelis-Menten Curve
50>
40-
30-
20-
+ W/o Inhibitor
10
-
+ With Inhibitor
0-
100
200
300
[S] (mM)
Velocity (umol/min)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fbd285f6f-67ec-4ffa-8e9b-700f12fa780d%2F273ade8a-5130-4e67-9e7e-8c15ced03085%2F7fx844_processed.jpeg&w=3840&q=75)
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