Calculate 1/[S] and 1/V to complete the table. Use this data to draw a Lineweaver-Burke plot, with lines for ‘no inhibitor’ and ‘with inhibitor’. Be sure to label your axes and lines. What kind of inhibitor is it? Estimate Vmax and Km for the uninhibited reaction.
Calculate 1/[S] and 1/V to complete the table. Use this data to draw a Lineweaver-Burke plot, with lines for ‘no inhibitor’ and ‘with inhibitor’. Be sure to label your axes and lines. What kind of inhibitor is it? Estimate Vmax and Km for the uninhibited reaction.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Calculate 1/[S] and 1/V to complete the table. Use this data to draw a Lineweaver-Burke plot, with lines for ‘no inhibitor’ and ‘with inhibitor’. Be sure to label your axes and lines.
What kind of inhibitor is it?
Estimate Vmax and Km for the uninhibited reaction.
![### Enzyme Kinetics Data
The following data was collected as part of a study on enzyme kinetics. The variables measured include substrate concentration \([S]\), reaction velocity \(V\) without and with an inhibitor, and their reciprocals. This type of data is often used to study the effects of inhibitors on enzymatic reactions and to determine kinetic parameters such as \(K_m\) and \(V_{max}\).
| \([S]\) | \(V\) (no inhibitor) | \(V\) (with inhibitor) | \(1/[S]\) | \(1/V\) (no \(I\)) | \(1/V\) (with \(I\)) |
|---------|-------------------------|--------------------|---------|-----------------|--------------------|
| 10 mM | 4 pmol sec\(^{-1}\) | 3 pmol sec\(^{-1}\) | | | |
| 20 mM | 8 pmol sec\(^{-1}\) | 5 pmol sec\(^{-1}\) | | | |
| 40 mM | 10 pmol sec\(^{-1}\) | 6 pmol sec\(^{-1}\) | | | |
Each row represents a different concentration of substrate \([S]\), with measured velocities \(V\) in the presence and absence of an enzyme inhibitor. The rightmost columns labeled \(1/[S]\), \(1/V\) (no \(I\)), and \(1/V\) (with \(I\)) typically contain the reciprocals, which are commonly used in Lineweaver-Burk plots for enzyme kinetics analysis. Note that these values still need to be calculated to complete the table.
This data can be used to analyze how the inhibitor affects enzyme activity, possibly altering the substrate affinity or the maximum reaction rate.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fb0d56cf4-ae0c-4387-a2fc-13a29f1b7e32%2Ffd73e95e-0f15-49ef-b1e4-ac358e96979d%2Fvcgvd7u_processed.png&w=3840&q=75)
Transcribed Image Text:### Enzyme Kinetics Data
The following data was collected as part of a study on enzyme kinetics. The variables measured include substrate concentration \([S]\), reaction velocity \(V\) without and with an inhibitor, and their reciprocals. This type of data is often used to study the effects of inhibitors on enzymatic reactions and to determine kinetic parameters such as \(K_m\) and \(V_{max}\).
| \([S]\) | \(V\) (no inhibitor) | \(V\) (with inhibitor) | \(1/[S]\) | \(1/V\) (no \(I\)) | \(1/V\) (with \(I\)) |
|---------|-------------------------|--------------------|---------|-----------------|--------------------|
| 10 mM | 4 pmol sec\(^{-1}\) | 3 pmol sec\(^{-1}\) | | | |
| 20 mM | 8 pmol sec\(^{-1}\) | 5 pmol sec\(^{-1}\) | | | |
| 40 mM | 10 pmol sec\(^{-1}\) | 6 pmol sec\(^{-1}\) | | | |
Each row represents a different concentration of substrate \([S]\), with measured velocities \(V\) in the presence and absence of an enzyme inhibitor. The rightmost columns labeled \(1/[S]\), \(1/V\) (no \(I\)), and \(1/V\) (with \(I\)) typically contain the reciprocals, which are commonly used in Lineweaver-Burk plots for enzyme kinetics analysis. Note that these values still need to be calculated to complete the table.
This data can be used to analyze how the inhibitor affects enzyme activity, possibly altering the substrate affinity or the maximum reaction rate.
Expert Solution
Step 1
A Lineweaver-Burk plot represents the relationship between the reciprocal values of substrate concentration and velocity. Lineweaver-Burk plot is a straight-line graph that helps to determine the Km and Vmax values. The Km and Vmax values are calculated by using the equation y=mx + b.
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