Homework Help is Here – Start Your Trial Now!
Science
Biology
Suppose in your biochemistry class you are given an enzyme and you are asked to determine whether egg or the starch is the substrate for this enzyme. Arrange the following statements such that it explains how you would go about doing this task from first step to the last step (you can drag and drop the choices to arrange from the first step to the last step): Drag and drop options into correct order and submit. For keyboard navigation. SHOW MORE V First take three test tubes labeled as A / B and C. A is the negative control and just add buffer. To the test-tube B add 5ml of raw egg in buffer. To tube C add 5 ml of starch solution in buffer After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg: To one set of tubes A/B and C add ninhydrine - appearance of = blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/ B and C add Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent = because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored II II

Suppose in your biochemistry class you are given an enzyme and you are asked to determine whether egg or the starch is the substrate for this enzyme. Arrange the following statements such that it explains how you would go about doing this task from first step to the last step (you can drag and drop the choices to arrange from the first step to the last step): Drag and drop options into correct order and submit. For keyboard navigation. SHOW MORE V First take three test tubes labeled as A / B and C. A is the negative control and just add buffer. To the test-tube B add 5ml of raw egg in buffer. To tube C add 5 ml of starch solution in buffer After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg: To one set of tubes A/B and C add ninhydrine - appearance of = blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/ B and C add Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent = because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored II II

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
icon
Concept explainers
Question
After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg : To one set of tubes A/B and Cadd ninhydrine - appearance of blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/B and Cadd Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored reaction Add 1 ml of enzyme to all the three test tubes A /Band Cand incubate at 37'C waterbath for 30 minutes II
Transcribed Image Text:After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg : To one set of tubes A/B and Cadd ninhydrine - appearance of blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/B and Cadd Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored reaction Add 1 ml of enzyme to all the three test tubes A /Band Cand incubate at 37'C waterbath for 30 minutes II
Suppose in your biochemistry class you are given an enzyme and you are asked to determine whether egg or the starch is the substrate for this enzyme. Arrange the following statements such that it explains how you would go about doing this task from first step to the last step (you can drag and drop the choices to arrange from the first step to the last step): Drag and drop options into correct order and submit. For keyboard navigation. SHOW MORE V First take three test tubes labeled as A / B and C. A is the negative control and just add buffer. To the test-tube B add 5ml of raw egg in buffer. To tube C add 5 ml of starch solution in buffer After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg : To one set of tubes A/B and Cadd ninhydrine - appearance of = blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/B and Cadd Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored II
Transcribed Image Text:Suppose in your biochemistry class you are given an enzyme and you are asked to determine whether egg or the starch is the substrate for this enzyme. Arrange the following statements such that it explains how you would go about doing this task from first step to the last step (you can drag and drop the choices to arrange from the first step to the last step): Drag and drop options into correct order and submit. For keyboard navigation. SHOW MORE V First take three test tubes labeled as A / B and C. A is the negative control and just add buffer. To the test-tube B add 5ml of raw egg in buffer. To tube C add 5 ml of starch solution in buffer After 30 minutes of incubation - take the three tubes out and divide the content of each tube into to two tubes. So you will now have total of 6 tubes - two for control - two 'B' tubes and two 'C' tubes First you will test to determine if the substrate is egg : To one set of tubes A/B and Cadd ninhydrine - appearance of = blue or brown or yellow color only in tube B which has egg confirms that the substrate for the given enzyme is egg (which is a protein). Next you will test to determine if the substrate is starch: To the other set of tubes A/B and Cadd Benedict's reagent = and heat it at 97'C for 10 minutes in a heat block. If you see appearance of yellow/red or orange colored solution in tube C then the substrate for the enzyme is starch. You should see appearance of yellow / orange/ red solution only in tube C after incubating with Benedict's reagent because the enzyme hydrolyzes starch into glucose and this glucose will then react with Benedict's reagent to give colored reaction You should see appearance of blue / yellow / brown solution only in tube B after adding Ninhydrin because the enzyme hydrolyzes egg (which is a protein) into amino acids which in turn react with Ninhydrin to give the colored II
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 2 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Knowledge Booster
DNA and RNA
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS