Is the data that you are collecting in the above table quantitative or qualitative? Explain why. Which treatment had the least amount of browning? Which had the most? Why do you think you obtained these results? Remember that the enzyme polyphenol oxidase is a protein! For each treatment, apply your knowledge of how temperature, pH, and salt concentration affect enzyme activity and explain why you got the results that you did. Include bonds and the levels of protein structure that you explored in Activity A in your answer. How does temperature impact the rate of enzyme activity? If you were to leave the apple in the refrigerator longer, why would it eventually brown? Explain based on what you know about enzyme activity. How does pH and its impact on specific types of bonds explain the results you obtained in your lemon juice treatment? Include bonds and levels of protein structure in your answer. How does salt and its impact on specific types of bonds explain the results you obtained in your salt treatment? Include bonds and levels of protein structure in your answer. How did the plastic wrap affect the browning reaction? Explain.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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  • Is the data that you are collecting in the above table quantitative or qualitative? Explain why.
  • Which treatment had the least amount of browning? Which had the most? Why do you think you obtained these results?
  • Remember that the enzyme polyphenol oxidase is a protein! For each treatment, apply your knowledge of how temperature, pH, and salt concentration affect enzyme activity and explain why you got the results that you did. Include bonds and the levels of protein structure that you explored in Activity A in your answer.
  • How does temperature impact the rate of enzyme activity? If you were to leave the apple in the refrigerator longer, why would it eventually brown? Explain based on what you know about enzyme activity.
  • How does pH and its impact on specific types of bonds explain the results you obtained in your lemon juice treatment? Include bonds and levels of protein structure in your answer.
  • How does salt and its impact on specific types of bonds explain the results you obtained in your salt treatment? Include bonds and levels of protein structure in your answer.
  • How did the plastic wrap affect the browning reaction? Explain.
Exercise D: Protein Structure and Enzyme Function
"Kitchen Lab"
** You will need to record your results over a 24 hour period for this lab**
Background:
Fruit turns brown when exposed to air because a reaction is happening when a cut
piece of fruit is exposed to oxygen. This is called enzymatic browning. The name
enzymatic browning comes from the fact that an enzyme located in the fruit reacts with
oxygen from the air to turn the fruit brown.
The general reaction can be simplified to:
Polyphenol oxidase + O2 → melanin (brown color)
Oxygen activates the compound polyphenol oxidase in the fruit to turn the fruit brown.
Polyphenol oxidase is the enzyme.
In this experiment you will perform a controlled experiment where you treat apple slices
in various conditions and gather data on how much the apple turned brown. If you do
not have the ingredients to conduct this experiment at home, see if you can reach out to
one of your classmates and borrow their data in order to analyze it. I will set up a
discussion board to help with this. Be sure to properly reference that individual.
For this experiment, use your information about bonds found in protein structure and be
able to hypothesize/predict what you may expect to see for the following treatments of
apple slices compared to the untreated control:
Adding lemon juice or vinegar
Adding salt
Covering with plastic wrap
Keeping at room temperature vs. in a refrigerator
If you're a parent, you might consider having your child(ren) help as a fun project!
Materials:
Apple
Knife
Lemon juice or vinegar
Salt water solution (approximately 1 teaspoon in 4 cup water)
Plastic wrap
Tongs or other utensil
Plates
Screenshot
Bowls
Transcribed Image Text:Exercise D: Protein Structure and Enzyme Function "Kitchen Lab" ** You will need to record your results over a 24 hour period for this lab** Background: Fruit turns brown when exposed to air because a reaction is happening when a cut piece of fruit is exposed to oxygen. This is called enzymatic browning. The name enzymatic browning comes from the fact that an enzyme located in the fruit reacts with oxygen from the air to turn the fruit brown. The general reaction can be simplified to: Polyphenol oxidase + O2 → melanin (brown color) Oxygen activates the compound polyphenol oxidase in the fruit to turn the fruit brown. Polyphenol oxidase is the enzyme. In this experiment you will perform a controlled experiment where you treat apple slices in various conditions and gather data on how much the apple turned brown. If you do not have the ingredients to conduct this experiment at home, see if you can reach out to one of your classmates and borrow their data in order to analyze it. I will set up a discussion board to help with this. Be sure to properly reference that individual. For this experiment, use your information about bonds found in protein structure and be able to hypothesize/predict what you may expect to see for the following treatments of apple slices compared to the untreated control: Adding lemon juice or vinegar Adding salt Covering with plastic wrap Keeping at room temperature vs. in a refrigerator If you're a parent, you might consider having your child(ren) help as a fun project! Materials: Apple Knife Lemon juice or vinegar Salt water solution (approximately 1 teaspoon in 4 cup water) Plastic wrap Tongs or other utensil Plates Screenshot Bowls
Procedures:
1. Prepare the solutions. Note that you only need a small amount of each, it is
sufficient to just wet the exposed surfaces of the apple, i.e., you may wet the one
side of the apple slice, flip it over, and then wet the other side.
2. Cut off both ends of the apple and then cut the apple into five slices (control,
lemon juice, salt, plastic wrap, refrigerator).
3. Set one untreated apple slice at room temperature (this is your control).
4. Set one untreated apple slice into your refrigerator.
5. Wrap one apple slice in plastic wrap; try and press the wrap closely against the
apple surface to exclude air.
6. Place one apple slice in the lemon juice or vinegar for 30 seconds.
7. Place one apple slice in the salt water solution for 30 seconds.
8. Be sure to label and keep track of each slice (e.g., place each on a paper platel
napkin, and write the treatment on the paper plate next to each slice).
9. Observe, photograph, and record your qualitative data after 10 minutes, 30
minutes, 1 hour, and 1 day in the table below. Use a scale of 0-5, where 0 is
unchanged, and 5 is very brown. Please hold on to this information as you will be
asked to submit it.
Table 1. Browning of apple slices over time for different treatments.
Apple slice
10 minutes
30 minutes
1 hour
24 hours
Untreated
1
1
2
control
Lemon juice or
vinegar
4
Salt water
1
Plastic wrap
1
1
1
1
Refrigerator
1
1
1
Reflection Questions:
Is the data that you are collecting in the above table quantitative or qualitative?
Explain why.
• Which treatment had the least amount of browning? Which had the most? Why
do you think you obtained
Screenshot
Transcribed Image Text:Procedures: 1. Prepare the solutions. Note that you only need a small amount of each, it is sufficient to just wet the exposed surfaces of the apple, i.e., you may wet the one side of the apple slice, flip it over, and then wet the other side. 2. Cut off both ends of the apple and then cut the apple into five slices (control, lemon juice, salt, plastic wrap, refrigerator). 3. Set one untreated apple slice at room temperature (this is your control). 4. Set one untreated apple slice into your refrigerator. 5. Wrap one apple slice in plastic wrap; try and press the wrap closely against the apple surface to exclude air. 6. Place one apple slice in the lemon juice or vinegar for 30 seconds. 7. Place one apple slice in the salt water solution for 30 seconds. 8. Be sure to label and keep track of each slice (e.g., place each on a paper platel napkin, and write the treatment on the paper plate next to each slice). 9. Observe, photograph, and record your qualitative data after 10 minutes, 30 minutes, 1 hour, and 1 day in the table below. Use a scale of 0-5, where 0 is unchanged, and 5 is very brown. Please hold on to this information as you will be asked to submit it. Table 1. Browning of apple slices over time for different treatments. Apple slice 10 minutes 30 minutes 1 hour 24 hours Untreated 1 1 2 control Lemon juice or vinegar 4 Salt water 1 Plastic wrap 1 1 1 1 Refrigerator 1 1 1 Reflection Questions: Is the data that you are collecting in the above table quantitative or qualitative? Explain why. • Which treatment had the least amount of browning? Which had the most? Why do you think you obtained Screenshot
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