Which property increases in magnitude during the steps of sequential purification of an enzyme? percent yield amount of total protein specific activity number of bands on a gel total enzyme activity

Q: Competitive and non-competitive inhibitions

A: Enzyme inhibition takes place when an inhibitor binds to the substrate and inhibit the activity of…

Q: nzyme - Acid Phosphatase Substrate - Para-nitrophenylphosphate Product - para-nitrophenol and…

A: Phosphatases are enzymes that catalyse the removal of the phosphate group from substrates.…

Q: the ability to carry out biochemical reactions is?

A: A biochemical reaction is such a chemical reaction that requires a specific biological catalyst…

Q: An immobilized enzyme plug flow reactor (PFR) contains a very porous honeycomb structure that has an…

A: The plug flow reactor model (PFR, sometimes called continuous tubular reactor, CTR, or piston flow…

Q: 979

A: Introduction:- There are two main types of nucleic acids - DNA and RNA. DNA acts as the genetic…

Q: ffinity labels in enzyme active sites explain

A: Enzymes are macromolecules that speed up the chemical reaction by reducing the barriers the affinity…

Q: Enzyme Combination - Cla I + EcoRI  What is the number of fragments? What are/is the size(s) of…

A: The restriction endonuclease are specific enzyme that are responsible for cutting double strength…

Q: isoenzyme, specific enzyme activity, what is it? Briefly explain.

A: Enzymes are proteins with a specific globular shape. Enzymes contain an active site to which a…

Q: Połymer formation from a pool of available monomers with a range of chemical functionalities…

A: Introduction:- The quetion is about the biomolecules that are polymers that made up of small units…

Q: Why understanding enzyme kinetics and inhibition of enzyme is serious business in modern…

A: Introduction Enzymes, biological catalysts, occur in biochemical reactions in all living organisms.…

Q: What are the (2) analytical methods would you suggest if your customer requested proof of purity of…

A: Introduction Enzymes are known as biocatalyst. Chemically enzymes are protein. Enzyme increases the…

Q: The oxyanion hole of a serine protease has which of the following roles (select all correct…

A: The objective of the question is to identify the roles of the oxyanion hole in a serine protease.…

Q: Dilution factor problem in buffer and unbuffered solution in enzyme.  In factors affecting enzyme…

A: In the Given question: 4 ml of 2% starch unbuffered solution was added to 4 ml of buffered solution…

Q: You need to make a 10 ng/ul solution of enzyme from a 100 mg/ml solution. How will you use a…

A: A serial dilution is the stepwise dilution of a substance in a solution. Where, the dilution factor…

Q: If an enzyme carries out acid-base catalysis, which of the following amino acids (in its…

A: Proteins are critical to the continued functioning of life on Earth. The vast majority of chemical…

Q: Consider the three-dimensional model of the tertiary structure of an enzyme below. Amino acids…

A: Introduction:- Proteins are made up of amino acids. They can be present in one-dimensional,…

Q: (d) From your plot, calculate the activity of the enzyme. Use the space below to show your workings:

A: Enzyme kinetics - is the study of the reaction rate of enzyme catalyzed reactions. The reaction rate…

Q: Cofactor Origin Structure Catalytic role Nicotinamide nucleotides Flavin nucleotides Adenosine…

A: Cofactor is the non-protein part of the enzyme. Cofactor is required for the biological activity of…

Q: H3. Draw the Lineweaver-Burk plots for the reaction that your selected enzyme is involved with and…

A: Inhibitors are substances which inhibit the activity of an enzyme. Inhibitors bind to the active…

Q: Which one is the most likely to be a catalytic triad of an enzyme that follows general acid base…

A: A catalytic triad is a set of three coordinated amino acids that can be found in the active site of…

Q: giving an overview of the use of colorimetric enzyme assays, as well as discussing their use in…

A: Colorimetric assays are reactions that lead to a change of color due to an enzymatic or chemical…

Q: How to find the optimum pH value at which an enzyme shows maximum activity.

A: Enzymes are proteins which accelerate the rate of a biochemical reaction. There are different…

Q: During successufl purification of every enzyme, the following may be expected: 1. Solubility in NaCl…

A: The specificity increases during the purification of every enzyme.

Q: Chemistry an enzyme binding equilibrium has a KD= 10 mm. What concentration of ligand (in mM) is…

A: Proteins that can act as biocatalysts for biochemical reactions are called enzymes. They are…

Q: Mechanistic probes often require the use of an electrophilic functional group to interact with an…

A: A nucleophile is a chemical species that is negatively charged or has a high electron density or a…

Q: Salting out and gelation of proteins is most advisable to carry out when biopolymers are in the…

A: Proteins are biomolecules made up of different amino acids. A protein becomes completely active in…

Q: In determining the activity of an enzyme of choice, which do you prefer, monitoring the product…

A: Enzymes are biological catalysts that enhance biochemical reactions in living organisms. The…

Q: Identify the biochemical building blocks that are the component of cell.

A: Energy is used by cells to carry out their essential tasks. Through the breakdown of organic…

Q: Estimate the concentration of an enzyme in a living cell. Assume that fresh tissue is 80% water and…

A: The catalytic amounts of the enzyme used under invitro conditions is in between 10-12-10-8 M. Fresh…

Q: Define optimum pH and temperature of an enzyme   How do changes in pH and temperature affect the…

A: A biochemical reaction is defined as the transformation of a reactant into a product (one molecule…

Q: During successful purification of every enzyme, the following may be expected: Select ALL that…

A: Enzyme purification and characterization is of great importance in obtaining information about…

Q: Understand when high specificity is important for an enzyme and how this specificity is achieved.

A: Substrate specificity is also known as absolute specificity which is very high for enzymes that…

Q: The enzyme catalase worked the best between pH 7 (optimum pH) and pH 11. What might be the cause of…

A: An enzyme is a catalytic molecule that increases the rate of any chemical reaction without being…

Q: Heating an enzyme beyond a certain point will cause it to denature. What will still be left when it…

A: DENATURATION It is the process modifying the molecular structure of an enzyme. Denaturation involves…

Q: 10. Using the following graph, explain how pH affects enzyme activity. x= optimal pH 8 10 PH Enzyme…

A: Enzymes are biological catalyst that speeds up the rate of a reaction by lowering its activation…

Q: Functional groups and linkages assignment Complete the reactions below. Circle and identify all…

A: The hydrocarbon compound is N-butylpropanamide which is reacting with water.

Q: A simple reaction in the laboratory can be complex in human body, give one example and discuss it

A: Human body is made of different  chemical molecules in which different chemical reactions takes…

Q: How do you calculate enzyme activity?

A: Enzymes are the biological catalysts that have the ability to alter the rate of metabolic reactions…

Q: A mutation alters a side chain on an enzyme involved with binding to the substrate, but not in…

A: A mutation is a change in the DNA sequence of a gene, chromosome, or entire genome. This can result…

Q: Match the structural biology method that is best suited to each task. obtaining a low-to-medium…

A: Structural biology is a branch of molecular biology, biochemistry, and biophysics that primarily…

Q: 1. If the Km of an enzyme for substrate A is 1 x 106 and for substrate B, is 4 x 10°, it means a.…

A: The Michaelis constant, Km, varies considerably from one enzyme to another, and also with a…

Q: Affinity purification: Assemble the following components to set up affinity chromatography to purify…

A: Affinity chromatography: It is a chromatographic technique for the separation of the compound on…