An immobilized enzyme plug flow reactor (PFR) contains a very porous honeycomb structure that has an enzyme immobilized on its surface. The substrate (S) enters the reactor at a concentration of 0.05 mole L-1along with an uncompetitive inhibitor (I) with a concentration of 0.01 mole L-1. The enzyme properties are such that: Vmax= 1.2 x 10-8mole cm-2sec-1, Km= 0.12 mole L-1. For the inhibitor KI= 0.05 mole L-1. The flowrate through the reactor is such that kL= 10-4cm sec-1. The surface area to volume ratio
Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
I2
An immobilized enzyme plug flow reactor (PFR) contains a very porous honeycomb structure that has an enzyme immobilized on its surface. The substrate (S) enters the reactor at a concentration of 0.05 mole L-1along with an uncompetitive inhibitor (I) with a concentration of 0.01 mole L-1. The enzyme properties are such that: Vmax= 1.2 x 10-8mole cm-2sec-1, Km= 0.12 mole L-1. For the inhibitor KI= 0.05 mole L-1. The flowrate through the reactor is such that kL= 10-4cm sec-1. The surface area to volume ratio of the honeycomb structure in the bioreactor is 1 cm-1. Estimate the residence time needed to achieve a 90 % conversion of the substrate. If the flowrate through the reactor is 100 L hr-1, what is the total volume (L) of the reactor needed to achieve this conversion? If the L/D ratio of the reactor is 10, what are the dimensions (ft) of the reactor? Repeat these calculations if external mass transfer effects are ignored.
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