1-In the first experiment, the researchers use a total enzyme concentration ([Et]) of 4 nM. They find that the happyase has a Vmax of 1.6 µs-1 in these conditions. Calculate the k2 of happyase based on this finding. Show your work and include appropriate units. 2-In another experiment, with [Et]=1 nM and [HAPPY]=30 µM, the researchers find that V0 = 300 nM.s-1. Calculate the Km of happyase for its substrate HAPPY based on this finding. Show your work and include appropriate units. 3-Further research shows that the purified happyase enzyme used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. After the careful removal of ANGER, the measured Vmax is increased to 4.8 µM.s-1, and the calculated Km becomes equal to 15 µM. Compare these values of Vmax and Km to that obtained in the experiments described in 1 and 2. To which category of reversible inhibitor ANGER belongs to? Explain.

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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A research group discovers a new enzyme they decide to name happyase. This enzyme has a Michaelian behavior and catalyzes the chemical reaction below:

HAPPY ↔ SAD

The researchers begin to characterize the enzyme.

1-In the first experiment, the researchers use a total enzyme concentration ([Et]) of 4 nM. They find that the happyase has a Vmax of 1.6 µs-1 in these conditions. Calculate the k2 of happyase based on this finding. Show your work and include appropriate units.

2-In another experiment, with [Et]=1 nM and [HAPPY]=30 µM, the researchers find that V0 = 300 nM.s-1. Calculate the Km of happyase for its substrate HAPPY based on this finding. Show your work and include appropriate units.

3-Further research shows that the purified happyase enzyme used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. After the careful removal of ANGER, the measured Vmax is increased to 4.8 µM.s-1, and the calculated Km becomes equal to 15 µM. Compare these values of Vmax and Km to that obtained in the experiments described in 1 and 2. To which category of reversible inhibitor ANGER belongs to? Explain.

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