A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of substrate concentration in the presence and absence of 0.001μM inhibitor. She obtains the following data. What is the Vmax and Km for the "no inhibitor" and "+ inhibitor" experiments. Ans, What would be value of V0 for the "+inhibitor" at [S] = 0.4μM and the inhibitor is most likely a competitive, uncompetitive or mixed inhibitor.
A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of substrate concentration in the presence and absence of 0.001μM inhibitor. She obtains the following data. What is the Vmax and Km for the "no inhibitor" and "+ inhibitor" experiments. Ans, What would be value of V0 for the "+inhibitor" at [S] = 0.4μM and the inhibitor is most likely a competitive, uncompetitive or mixed inhibitor.
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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A researcher has measured the initial rate of an enzyme-catalyzed reaction as a function of substrate concentration in the presence and absence of 0.001μM inhibitor. She obtains the following data.
What is the Vmax and Km for the "no inhibitor" and "+ inhibitor" experiments. Ans, What would be value of V0 for the "+inhibitor" at [S] = 0.4μM and the inhibitor is most likely a competitive, uncompetitive or mixed inhibitor.
![substrate concer
lowing data.
[S] (UM)
27
0.005
0.01
0.15
0.18
0.2
0.5
2
5
20
50
100
V, (μMS-¹)
No Inhibitor
6.0
13.0
25.0
50.0
71.0
91.0
96.0
99.0
100.0
100.0
100.0
+ Inhibitor
1.0
3.0
6.0
15.0
23.0
50.0
72.0
89.0
98.0
100.0
100.0](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F95cd45c3-2607-4ce9-ae8f-b44cec02c742%2F0614969a-ebb6-487d-8604-963eecb11e9a%2F2i487u4m_processed.jpeg&w=3840&q=75)
Transcribed Image Text:substrate concer
lowing data.
[S] (UM)
27
0.005
0.01
0.15
0.18
0.2
0.5
2
5
20
50
100
V, (μMS-¹)
No Inhibitor
6.0
13.0
25.0
50.0
71.0
91.0
96.0
99.0
100.0
100.0
100.0
+ Inhibitor
1.0
3.0
6.0
15.0
23.0
50.0
72.0
89.0
98.0
100.0
100.0
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