Aldolase enzymes have the following rate constants. k₁ = 10³M ¹s¹, k-₁ = 4.4x10 s ¹, k₂ = 10's-¹ -1 Enzyme concentration is 10 6 M and substrate concentration is 10-³M (a) Use the assumption of Michaelis and Menten to find the rate of enzyme reaction (b) As a substance (1) was added, the enzyme reaction was reversibly inhibited, and the lineweaver-Burk plot changed as follows. When [1]=2KI, find the reaction rate. 1/v [0]=0 1/[S]
Aldolase enzymes have the following rate constants. k₁ = 10³M ¹s¹, k-₁ = 4.4x10 s ¹, k₂ = 10's-¹ -1 Enzyme concentration is 10 6 M and substrate concentration is 10-³M (a) Use the assumption of Michaelis and Menten to find the rate of enzyme reaction (b) As a substance (1) was added, the enzyme reaction was reversibly inhibited, and the lineweaver-Burk plot changed as follows. When [1]=2KI, find the reaction rate. 1/v [0]=0 1/[S]
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4
![Aldolase enzymes have the following rate constants.
-1
k₁ = 10 M¹s¹, k-₁ = 4.4×10's-¹, k₂ = 10's-¹
S
Enzyme concentration is 106M and substrate
concentration is 10-³M
(a) Use the assumption of Michaelis and Menten to find
the rate of enzyme reaction
(b) As a substance (1) was added, the enzyme reaction
was reversibly inhibited, and the lineweaver-Burk plot
changed as follows. When [1]=2KI, find the reaction
rate.
1/v
(1)=0
1/[S]](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F54fb1c47-3135-49d2-a4da-da95179ef74a%2F1156c66c-8630-4c7b-900c-d83b9582ae5b%2Fcxpzn1_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Aldolase enzymes have the following rate constants.
-1
k₁ = 10 M¹s¹, k-₁ = 4.4×10's-¹, k₂ = 10's-¹
S
Enzyme concentration is 106M and substrate
concentration is 10-³M
(a) Use the assumption of Michaelis and Menten to find
the rate of enzyme reaction
(b) As a substance (1) was added, the enzyme reaction
was reversibly inhibited, and the lineweaver-Burk plot
changed as follows. When [1]=2KI, find the reaction
rate.
1/v
(1)=0
1/[S]
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