Homework Help is Here – Start Your Trial Now!
Science
Biology
blem asks students to think about enzyme kinetics in the framework of the Michaelis-Menten equation. It asks students to consider experiments to determine values for Km and Vmax and to draw graphs showing that they understand the concepts of Km and Vmax. It also requires students to understand what the units involved in such experiments are and what they mean (necessary to put correct units on the graph axes). 1. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20> H+ + HCO3 Important: Assume the Km of one variant of carbonic anhydrase is 1 µM. First, imagine an experiment employing 10 test tubes. The test tubes are all filled with 100 ml water. Imagine that the different test tubes have different concentrations of CO2, ranging from 0.1 µM to 10 mM CO2. Initially, there is NO enzyme in any of the test tubes. (a) Draw a graph showing what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Be sure to label the axes of the graph. Designate this curve as "A" (note: the exact values on the Y axes here are questimates you get to make them un)

blem asks students to think about enzyme kinetics in the framework of the Michaelis-Menten equation. It asks students to consider experiments to determine values for Km and Vmax and to draw graphs showing that they understand the concepts of Km and Vmax. It also requires students to understand what the units involved in such experiments are and what they mean (necessary to put correct units on the graph axes). 1. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2 + H20> H+ + HCO3 Important: Assume the Km of one variant of carbonic anhydrase is 1 µM. First, imagine an experiment employing 10 test tubes. The test tubes are all filled with 100 ml water. Imagine that the different test tubes have different concentrations of CO2, ranging from 0.1 µM to 10 mM CO2. Initially, there is NO enzyme in any of the test tubes. (a) Draw a graph showing what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Be sure to label the axes of the graph. Designate this curve as "A" (note: the exact values on the Y axes here are questimates you get to make them un)

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
See similar textbooks
icon
Related questions
Question
Physiology Michaelis Menten relations
Worksheet #2 Michaelis-Menten relations The following problem asks students to think about enzyme kinetics in the framework of the Michaelis-Menten equation. It asks students to consider experiments to determine values for Km and Vmax and to draw graphs showing that they understand the concepts of Km and Vmax. It also requires students to understand what the units involved in such experiments are and what they mean (necessary to put correct units on the graph axes). 1. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2+ H20> H+ + HCO3 Important: Assume the Km of one variant of carbonic anhydrase is 1 µM. First, imagine an experiment employing 10 test tubes. The test tubes are all filled with 100 ml water. Imagine that the different test tubes have different concentrations of CO2, ranging from 0.1 μM to 10 mM CO2. Initially, there is NO enzyme in any of the test tubes. (a) Draw a graph showing what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Be sure to label the axes of the graph. Designate this curve as "A" (note: the exact values on the Y axes here are guestimates - you get to make them up). (b) Now, imagine that the test tubes are filled with water and a significant amount of the enzyme carbonic anhydrase in each test tube. Assume that the enzyme has a Km of 1 µM. Draw on the same graph as before what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Label this curve "B". (note: again, the exact values on the Y axes here are guestimates - you get to make them up). (c) Now, on the same plot, draw a graph showing the curve that results when you subtract the data obtained in the first condition (without enzyme) from the data obtained in the experiments obtained when the enzyme is present. Label this curve "C".. (d). Using the data from C above (that is, subtracting the background production of CO2 due to simple mass action from that resulting from the combination of enzyme action and mass action), draw a separate graph showing the alteration in the rate of carbonic anhydrase as the CO2 level is varied as a percentage of the maximal rate of HCO3 production that is, 100% is as fast as the enzyme can function. Be sure to label the axes of the graphs. That is, again draw a graph showing what you might expect to see for the amount of HCO3 produced by the action of of the enzyme in each of the test tubes 1 minute after the addition of CO2. LED TO 2
Transcribed Image Text:Worksheet #2 Michaelis-Menten relations The following problem asks students to think about enzyme kinetics in the framework of the Michaelis-Menten equation. It asks students to consider experiments to determine values for Km and Vmax and to draw graphs showing that they understand the concepts of Km and Vmax. It also requires students to understand what the units involved in such experiments are and what they mean (necessary to put correct units on the graph axes). 1. Carbonic anhydrase is an enzyme that will convert CO2 and water into HCO3. CO2+ H20> H+ + HCO3 Important: Assume the Km of one variant of carbonic anhydrase is 1 µM. First, imagine an experiment employing 10 test tubes. The test tubes are all filled with 100 ml water. Imagine that the different test tubes have different concentrations of CO2, ranging from 0.1 μM to 10 mM CO2. Initially, there is NO enzyme in any of the test tubes. (a) Draw a graph showing what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Be sure to label the axes of the graph. Designate this curve as "A" (note: the exact values on the Y axes here are guestimates - you get to make them up). (b) Now, imagine that the test tubes are filled with water and a significant amount of the enzyme carbonic anhydrase in each test tube. Assume that the enzyme has a Km of 1 µM. Draw on the same graph as before what you might expect to see for the amount of HCO3 in each of the test tubes 1 minute after the addition of CO2. Label this curve "B". (note: again, the exact values on the Y axes here are guestimates - you get to make them up). (c) Now, on the same plot, draw a graph showing the curve that results when you subtract the data obtained in the first condition (without enzyme) from the data obtained in the experiments obtained when the enzyme is present. Label this curve "C".. (d). Using the data from C above (that is, subtracting the background production of CO2 due to simple mass action from that resulting from the combination of enzyme action and mass action), draw a separate graph showing the alteration in the rate of carbonic anhydrase as the CO2 level is varied as a percentage of the maximal rate of HCO3 production that is, 100% is as fast as the enzyme can function. Be sure to label the axes of the graphs. That is, again draw a graph showing what you might expect to see for the amount of HCO3 produced by the action of of the enzyme in each of the test tubes 1 minute after the addition of CO2. LED TO 2
Expert Solution
steps

Step by step

Solved in 5 steps

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:
9780134580999
Author:
Elaine N. Marieb, Katja N. Hoehn
Publisher:
PEARSON
Biology 2e
Biology 2e
Biology
ISBN:
9781947172517
Author:
Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:
OpenStax
Anatomy & Physiology
Anatomy & Physiology
Biology
ISBN:
9781259398629
Author:
McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:
Mcgraw Hill Education,
Molecular Biology of the Cell (Sixth Edition)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:
9780815344322
Author:
Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:
W. W. Norton & Company
Laboratory Manual For Human Anatomy & Physiology
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:
9781260159363
Author:
Martin, Terry R., Prentice-craver, Cynthia
Publisher:
McGraw-Hill Publishing Co.
Inquiry Into Life (16th Edition)
Inquiry Into Life (16th Edition)
Biology
ISBN:
9781260231700
Author:
Sylvia S. Mader, Michael Windelspecht
Publisher:
McGraw Hill Education
SEE MORE TEXTBOOKS