Milli absorbance unit (mAU) 9) Draw a standard curve using the chromatogram with molecular weight values of blue peaks given in kDa in the graph below. Estimate the MW (in kDa) of the proteins in red peaks A-D on the overlayed chromatogram. (20 points) MAU 200- 175- 150- 125- 100- 75 50- 25 0 2000 669 43 33 29 440 158 13.7 10 A B 15 C Retention time (minutes) 20 D 25 30

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