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Amino Acid Molecular Weight 137 Alanine 89 Arginine 174 Aspartic acid 133 Glutamic acid 146 194 Glycine 75 Lysine 146 105 Proline 115 146 Serine 105 Tyrosine 181 Valine 117 340 384 425. 473 Hab 279 + 530 + 619 Mass/charge (here the charge is -1, so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this pepetide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) The masses given should be less by one (loss of H ion to impose a charge on the molecules). To avoid any possible confusion, I left that out.

Amino Acid Molecular Weight 137 Alanine 89 Arginine 174 Aspartic acid 133 Glutamic acid 146 194 Glycine 75 Lysine 146 105 Proline 115 146 Serine 105 Tyrosine 181 Valine 117 340 384 425. 473 Hab 279 + 530 + 619 Mass/charge (here the charge is -1, so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this pepetide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) The masses given should be less by one (loss of H ion to impose a charge on the molecules). To avoid any possible confusion, I left that out.

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Biochemistry Four - Mass Spectroscopy Question. Please see image

Amino Acid Molecular Weight 137 Alanine 89 Arginine 174 Aspartic acid 133 Glutamic acid 146 194 Glycine 75 Lysine 146 105 Proline 115 146 Serine 105 Tyrosine 181 Valine 117 340 384 425. 473 Hab 279 + 530 + 619 Mass/charge (here the charge is -1, so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this pepetide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) The masses given should be less by one (loss of H ion to impose a charge on the molecules). To avoid any possible confusion, I left that out.
Transcribed Image Text:Amino Acid Molecular Weight 137 Alanine 89 Arginine 174 Aspartic acid 133 Glutamic acid 146 194 Glycine 75 Lysine 146 105 Proline 115 146 Serine 105 Tyrosine 181 Valine 117 340 384 425. 473 Hab 279 + 530 + 619 Mass/charge (here the charge is -1, so it's just mass) Above is shown a mass spectroscopy for a peptide 5 amino acids long derived from a soluble protein. This 5 amino acid peptide represents a hydrophilic surface part of the protein (this is a hint as to the composition of this pepetide). Determine and give the amino acid sequence of this pentapeptide. Show which end is the amino terminus and which is the carboxy terminus. NOTE: (1) for simplicities sake not all possible peaks are shown, though all relevant peaks are. (2) Not all amino acids are listed, but all relevant/necessary amino acids are. (3) The masses given should be less by one (loss of H ion to impose a charge on the molecules). To avoid any possible confusion, I left that out.
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