3. You are studying a protein that has a molar extinction coefficient of 1358 M-1 cm-1. You measure a solution of this protein using a 1 cm cuvette and find that the absorbance is 0.47. What is the concentration of the protein? Which amino acids are likely found in this protein? If you thought the protein of interest was pure, but there are actually some contaminants remaining from the purification process, will you be overestimating or underestimating the concentration of your protein of interest?

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