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7) You will use about 1 ml of the following buffer every day in the lab so you decide to make a 5X master mix that will last for a year. List the stock solutions you will make and how you will make the stocks (also how much). Then list how you will dilute them to make the 5X master mix (and how much). We don't want to waste materials. (25 points) 25 mM Tris buffer, pH 8.0 50 mM sodium chloride 2 mM NaEDTA 5 mM dithiothreitol (DTT) 10 mM MgCl2 0.1% CHAPS Final Stock concentration solutions 25 mM Tris 1 M. 2 mM NaEDTA 250 mM 1 M 5 mM DTT 50 mM NaCl 1 M 10 mM MgCl2 1.5 M 0.1%CHAPS 10% (w/v) Totals (mLs) (5X) Master Fraction of dilution needed concentration from Stock to 5X MM Volume Needed (mL) for 50 mL 5X Solid grams Mix to make 100 ml of stock MM

7) You will use about 1 ml of the following buffer every day in the lab so you decide to make a 5X master mix that will last for a year. List the stock solutions you will make and how you will make the stocks (also how much). Then list how you will dilute them to make the 5X master mix (and how much). We don't want to waste materials. (25 points) 25 mM Tris buffer, pH 8.0 50 mM sodium chloride 2 mM NaEDTA 5 mM dithiothreitol (DTT) 10 mM MgCl2 0.1% CHAPS Final Stock concentration solutions 25 mM Tris 1 M. 2 mM NaEDTA 250 mM 1 M 5 mM DTT 50 mM NaCl 1 M 10 mM MgCl2 1.5 M 0.1%CHAPS 10% (w/v) Totals (mLs) (5X) Master Fraction of dilution needed concentration from Stock to 5X MM Volume Needed (mL) for 50 mL 5X Solid grams Mix to make 100 ml of stock MM

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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7) You will use about 1 ml of the following buffer every day in the lab so you decide to make a 5X master mix that will last for a year. List the stock solutions you will make and how you will make the stocks (also how much). Then list how you will dilute them to make the 5X master mix (and how much). We don't want to waste materials. (25 points) 25 mM Tris buffer, pH 8.0 50 mM sodium chloride 2 mM NaEDTA 5 mM dithiothreitol (DTT) 10 mM MgCl2 0.1% CHAPS Final Stock concentration solutions 25 mM Tris 1 M. 2 mM NaEDTA 250 mM 1 M 5 mM DTT 50 mM NaCl 1 M 10 mM MgCl2 1.5 M 0.1%CHAPS 10% (w/v) Totals (mLs) (5X) Master Fraction of dilution needed concentration from Stock to 5X MM Volume Needed (mL) for 50 mL 5X Solid grams Mix to make 100 ml of stock MM
Transcribed Image Text:7) You will use about 1 ml of the following buffer every day in the lab so you decide to make a 5X master mix that will last for a year. List the stock solutions you will make and how you will make the stocks (also how much). Then list how you will dilute them to make the 5X master mix (and how much). We don't want to waste materials. (25 points) 25 mM Tris buffer, pH 8.0 50 mM sodium chloride 2 mM NaEDTA 5 mM dithiothreitol (DTT) 10 mM MgCl2 0.1% CHAPS Final Stock concentration solutions 25 mM Tris 1 M. 2 mM NaEDTA 250 mM 1 M 5 mM DTT 50 mM NaCl 1 M 10 mM MgCl2 1.5 M 0.1%CHAPS 10% (w/v) Totals (mLs) (5X) Master Fraction of dilution needed concentration from Stock to 5X MM Volume Needed (mL) for 50 mL 5X Solid grams Mix to make 100 ml of stock MM
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