You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTAS kinetics data are shown below: [Substrate, uM] Vo with DEDS M/min) Vo without DEDS (uM/min) 13.333 0.774 1.196 4.000 0.877 1.316 THE 5.000 1.012 1.462 10.000 1.462 1.880 20.000 2.193. 088
You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTAS kinetics data are shown below: [Substrate, uM] Vo with DEDS M/min) Vo without DEDS (uM/min) 13.333 0.774 1.196 4.000 0.877 1.316 THE 5.000 1.012 1.462 10.000 1.462 1.880 20.000 2.193. 088
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTASE, The
kinetics data are shown below
[Substrate, uM]
Vo with DEDS M/min)
Vo without DEDS (uM/min)
M3.333
10.774,
11.196
14,000
0.877
1.316
5.000
1.012
#1.462
10.000
1.462
088'1
20.000
1.880
12198
Using linear regression analysis. deterane tievaluesof Vun K orthe ercymehine ABSENCE of inhibitoe Express your answer
In 3 decimal places, do NOT include the units)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8babc06a-d687-4abd-bcd5-fd898b2485c7%2F95471442-5ec5-4333-86fc-664f561c9da5%2Fgjeahhc_processed.jpeg&w=3840&q=75)
Transcribed Image Text:You were asked to determine the mode of inhibition exerted by Inhibitor DEDS to a newly discovered enzyme known as BILISTASE, The
kinetics data are shown below
[Substrate, uM]
Vo with DEDS M/min)
Vo without DEDS (uM/min)
M3.333
10.774,
11.196
14,000
0.877
1.316
5.000
1.012
#1.462
10.000
1.462
088'1
20.000
1.880
12198
Using linear regression analysis. deterane tievaluesof Vun K orthe ercymehine ABSENCE of inhibitoe Express your answer
In 3 decimal places, do NOT include the units)
![Using linear regression analysis, determine the values of V and Ky of the enzyme in the PRESENCE ofinhibitor: (5
in 3 decimal places, do NOT incude the units)
Vmax
KM=
What is the MODE of inhibition exerted by DEDS?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F8babc06a-d687-4abd-bcd5-fd898b2485c7%2F95471442-5ec5-4333-86fc-664f561c9da5%2F1411q1_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Using linear regression analysis, determine the values of V and Ky of the enzyme in the PRESENCE ofinhibitor: (5
in 3 decimal places, do NOT incude the units)
Vmax
KM=
What is the MODE of inhibition exerted by DEDS?
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