4 Dichlorophenol (the substrate) is degraded by an enzyme with the following data collected for different concentrations of substrate: Initial Substrate Concentration, S. (mg/L) 10 20 30 40 50 67 83 Reaction Rate, v (mg product/L-h) 3.86 6.99 10.5 13.6 15.6 13.1 8.28 100 4.6 A. By examining the data, or plotting v vs. [S], determine what type of inhibition is occurring for this reaction. B. Make a Lineweaver-Burk plot for the portion of the data that matches Michaelis-Menten behavior and determine Km and Vmax. C. Does the reaction reach Vmax for any of the [S] studied?
4 Dichlorophenol (the substrate) is degraded by an enzyme with the following data collected for different concentrations of substrate: Initial Substrate Concentration, S. (mg/L) 10 20 30 40 50 67 83 Reaction Rate, v (mg product/L-h) 3.86 6.99 10.5 13.6 15.6 13.1 8.28 100 4.6 A. By examining the data, or plotting v vs. [S], determine what type of inhibition is occurring for this reaction. B. Make a Lineweaver-Burk plot for the portion of the data that matches Michaelis-Menten behavior and determine Km and Vmax. C. Does the reaction reach Vmax for any of the [S] studied?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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![4
Dichlorophenol (the substrate) is degraded by an enzyme with the following data collected for different
concentrations of substrate:
Initial Substrate Concentration, S. (mg/L)
10
20
30
40
50
67
83
Reaction Rate, v (mg product/L-h)
3.86
6.99
10.5
13.6
15.6
13.1
8.28
100
4.6
A. By examining the data, or plotting v vs. [S], determine what type of inhibition is occurring for this reaction.
B. Make a Lineweaver-Burk plot for the portion of the data that matches Michaelis-Menten behavior and
determine Km and Vmax.
C. Does the reaction reach Vmax for any of the [S] studied?](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F28accb41-ca20-496f-8540-0972758d5e2c%2F00810a10-b7ae-4e3b-982d-08049eef5df1%2Fkunwzx1_processed.jpeg&w=3840&q=75)
Transcribed Image Text:4
Dichlorophenol (the substrate) is degraded by an enzyme with the following data collected for different
concentrations of substrate:
Initial Substrate Concentration, S. (mg/L)
10
20
30
40
50
67
83
Reaction Rate, v (mg product/L-h)
3.86
6.99
10.5
13.6
15.6
13.1
8.28
100
4.6
A. By examining the data, or plotting v vs. [S], determine what type of inhibition is occurring for this reaction.
B. Make a Lineweaver-Burk plot for the portion of the data that matches Michaelis-Menten behavior and
determine Km and Vmax.
C. Does the reaction reach Vmax for any of the [S] studied?
Expert Solution
Step 1
Rate of enzyme reaction is measured by the rate at which substrate is used up or product is formed. Generally, rate of reaction is increased due to increase in enzyme or substrate concentration, temperature etc. However, presence of inhibitors can effect the enzyme reaction rate.
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