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Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 5 1/[S], (L/mol)

Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 5 1/[S], (L/mol)

Biochemistry
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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4. Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 1/[S], (L/mol) 5
Transcribed Image Text:4. Lineweaver-Burk plot determined an equation for the trendline linear curve fit to be y = 4.20 x + 18.3 for isomerization of glucose using glucose isomerase. Using a spreadsheet (computer), reconstruct the reaction velocity vs. substrate concentration graph. Be sure to include units on your graph. 1/v, (L-s/mol) 50 45 40 35 30 25 20 15 10 5 0 0 0.5 Lineweaver-Burk Plot 1 1.5 2 2.5 3 3.5 4 4.5 1/[S], (L/mol) 5
Expert Solution
Step 1

The line weaver burk plot is a double reciprocal plot, i.e., it is the graph for 1V vs 1[S],

where V is the enzyme velocity, and [S] is the substrate concentration. 

 

The linear curve given fits the equation: y=4.20x+18.3, where X represents 1[S] and Y represents 1V,

 

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