The hydrolysis of a substrate, S, by an enzyme has been studied in the lab. The following initial rates, vo,were recorded at different concentrations.[S] (M)Vo (M/min)2.10-10-41.20.10-64.20-10-43.10.10-69.30-10-46.30-10-61.42-10-39.10-10-6A. Determine the rate constants for degradation of the substrateB. What is the rate of reaction at [S] = 1.1.104 M?C. Explain, why enzymes can make reactions go faster? Does enzymes also catalyse the reversereaction from product to substrate?

Consider the reaction where a substrate 'S' is converted to its product 'P' by an enzyme. The rate…

Answered: The hydrolysis of a substrate, S, by an…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
Please answer in full.
8 7- 6 2- 180 225 270 315 [Substrate] (nM) 45 90 135 360 405 450 495 540 58! What is the KM of the enzyme data shown above? rate (nM/sec) 3.
Use the graph below to determine which of the following is the optimal temperature for catalase. Catalase Activity 1.5 1.4 1.3 1.2 1.1 1.0 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 0.1 10 20 30 40 50 60 Temperature (°C) O 0.8 O 1.45 O 37 O 20 Percent Increase in Oxygen per Minute
What percentage of max is obtained when the substrate is present at 80% of the Km? Use two digits in your answer.
When reviewing a Michaelis-Menten Saturation Curve, at first the rate of the reaction is relatively constant, but the rate decreases as the substrate is used up and eventually reaches a plateau. Afte reaching this plateau, what would speed up the reaction again? Adding more substrate Adding heat Adding more enzyme Adding cofactors
The following data were collected in the study of a new enzyme and an inhibitor of the new enzyme: Vo (nmol/sec) [S] (uM) 1.3 - Inhibitor + Inhibitor 2.50 0.62 2.6 4.00 1.42 6.5 6.30 2.65 13.0 7.60 3.12 26.0 9.00 3.58 What is the Km of the inhibited enzyme reaction?
2.2 An investigation was carried out to determine the effect of a detergent containing fat-digesting enzymes on a fat staln. The procedure was as follows: Two identical cloths were used. Cloths A and B had identical fat stains. Cloth A was washed using a detergent not containing fat-digesting enzymes for five minutes at 37°C. Cloth B was washed using a detergent containing fat-digesting enzymes for 2.3 five minutes at 37°C. The diagram below shows the results of the investigation. Cloth A Cloth B Fat stain (2 2.2.1 State a conclusion for this investigation. State TWO factors that were kept constant during this investigation. 2.2.2 State what should have happened to the enzymes if cloth B was washed in boiling water. 2.2.3 What was the purpose of cloth A in the investigation? 2.2.4
You run 5 standard proteins listed below on a size-exclusion (gel filtration) column with limit of 200,000Da. Please draw a chromatogram on a separate page with each peak and both axes labeled. Protein B-Amylase Alcohol dehydrogenase Bovine albumin Carbonic anhydrase Cytochome A280 MW 223,800 0.5 82,000 0.6 66,463 0.45 30,000 0.43 12,000 0.8
Consider the proteins in the table below. Protein Isoelectric point (pl) М.W. Alcohol dehydrogenase 150,000 5.5 Cytochrome C 12,400 10.5 Serum albumin 66,000 5.3 B-Amylase 200,000 5.7 Apoferitin 443,000 4.0 Fill in the blank. If the mixture of proteins above was subjected to anion exchange chromatography at pH 8, from the column. would be the first protein to elute
What is the initial velocity measurement for the hydrolysis of 50 μM amoxicillin by the enzyme?
Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patient

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