The hydrolysis of a substrate, S, by an enzyme has been studied in the lab. The following initial rates, vo,were recorded at different concentrations.[S] (M)Vo (M/min)2.10-10-41.20.10-64.20-10-43.10.10-69.30-10-46.30-10-61.42-10-39.10-10-6A. Determine the rate constants for degradation of the substrateB. What is the rate of reaction at [S] = 1.1.104 M?C. Explain, why enzymes can make reactions go faster? Does enzymes also catalyse the reversereaction from product to substrate?

Consider the reaction where a substrate 'S' is converted to its product 'P' by an enzyme. The rate…

Answered: The hydrolysis of a substrate, S, by an…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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3L.6.2
You want to prepare 500 mL of the macronutrient and micronutrient stock solutions which have 100X and 1000X higher concentration, respectively, than the required concentration in the culture mediuma)If 0.17 g/L KH2PO4 is required in MS medium, determine the mass (g) of KH2PO4 that is required to prepare the stock solution and the volume required (mL) to prepare 200 mL MS medium. Show your work.
You run 5 standard proteins listed below on a size-exclusion (gel filtration) column with limit of 200,000Da. Please draw a chromatogram on a separate page with each peak and both axes labeled. Protein B-Amylase Alcohol dehydrogenase Bovine albumin Carbonic anhydrase Cytochome A280 MW 223,800 0.5 82,000 0.6 66,463 0.45 30,000 0.43 12,000 0.8
Consider the proteins in the table below. Protein Isoelectric point (pl) М.W. Alcohol dehydrogenase 150,000 5.5 Cytochrome C 12,400 10.5 Serum albumin 66,000 5.3 B-Amylase 200,000 5.7 Apoferitin 443,000 4.0 Fill in the blank. If the mixture of proteins above was subjected to anion exchange chromatography at pH 8, from the column. would be the first protein to elute
Urine samples for each patient were tested for ketone bodies, and a Benedict's reagent and glucose oxidase reaction was also completed. The results are given in the table below, with - indicating a negative result and +, ++ and +++ indicating a positive result of different intensity. Patient A Patient B Patient C Patient D Patient E Ketone Bodies +++ Benedict's Reagent +++ - +++ - +++ Glucose Oxidase +++ +++ + 4. For each individual patient A-E, using the WHO guidelines for the diagnosis of diabetes, write a short paragraph to explain: (a) what you can deduce from the fasting blood glucose and the 2 hour sample of that patient
explain the results of this tables
I have successfully extracted crude enzymes from the cocoa pod husk, an agriculture waste. (a)The enzyme has optimum activities at 35oC and pH 6.5. With the correct captions for X and Y axes, help me to draw: A temperature profile graph, from 20 to 70oC. A pH profile graph from 2 to 12.
You can use this as your references: https://www.youtube.com/watch?v=34VkaCJggOM
3. Compute for the amount of each component of KCN broth if you were to prepare 280 ml. Express your answers using two decimal places. Follow this format in typing your answers: answerabbreviated unit (e.g. 3.00 g, 0.75 ml, 10.25 mg): Components Amount per liter Polypeptone 3.0 g N22HPO4 5.64 g (Available as NazHPO4 · 7H2O*) Monopotassium phosphate 0.225 g Sodium chloride 0.5% KCN 0.075 g (Available as 10,000 ppm solution) *MW (g/mol): Na-23; P-31; H-1; 0-16 3.3. Monopotassium phosphate /r
You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2 μM substrate. At this substrate concentration, you determine that the Vo = 88.6 μmol mL-¹. s-¹. If the Vmax 833.3 mM s the KM is: . == " 30.9 μΜ 10.4 μΜ Ο 124.6 μΜ 234 μΜ 94.5 μM
A purified protein fraction has a total sample volume of 360 µL. The sample has a corrected A280 of 0.484, and the blank corrected A280 was 0.052. (Both values were measured with a path length of 1.00 cm.) If 5.00 µL of the sample was used in a reaction, calculate the mass of protein in the reaction (in µg).
A patient is prescribed Cipro for an upper espiratory tract infection. The oral suspension is labelled 200 mg/5 mL and the patient needs to take 800 mg every 12 hours for 7 to 15 days. How many ug are in each dose? How many mL must the patient take in each dose? How many g of Cipro is the patient taking every 24 hours? What is the molarity of the Cipro oral suspension? MW Cipro=331.35 g/mole

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