For the reaction A ==> B, Delta G'° = -60 kJ/mol. The reaction is started with 10 mmol of A;no B is initially present. After 24 hours, analysis reveals the presence of 2 mmol of B, 8 mmolof A. Which is the most likely explanation?OA) A and B have reached equilibrium concentrationsB) An enzyme has shifted the equilibrium toward AC) B formation is kinetically slow; equilibrium has not been reached by 24 hours○ D) Formation of B is thermodynamically unfavorable○ The result described is impossible, given that Delta G'o is -60 kJ/mol

Answered: For the reaction A ==> B, Delta G'° =…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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For an enzyme kinetics experiment, a student prepared a reaction mixture by mixing 450 microliters of 0.75mM PNPP with 4.25ml of 0.2M Tris-HCl buffer. When he is ready to measure the absorbance, he added 0.3ml of Alkaline Phosphatase to the mixture and mixed thoroughly. What is the substrate concentration at the beginning of the reaction in mM ?
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