A receptor, R, binds to its ligand, L, according to the binding curve shown below. If [R]= 30 mM at the KD, what is the concentration of the receptor-ligand complex at the KD? 1.0 Q2 10 15 20 25 30 35 40 45 50 55 60 0 0.5 40 mM O 25 mM O 30 mM O 10 mM 17.5 ml 5 [Ligand] (mm)

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
icon
Related questions
Question
A receptor, R, binds to its ligand, L, according to the binding curve shown below. If
[R]= 30 mM at the KD, what is the concentration of the receptor-ligand complex at
the KD?
1.0
Q2
10 15 20 25
30 35 40 45 50 55 60
0 0.5
40 mM
O 25 mM
O 30 mM
O 10 mM
17.5 ml
5
[Ligand] (mm)
Transcribed Image Text:A receptor, R, binds to its ligand, L, according to the binding curve shown below. If [R]= 30 mM at the KD, what is the concentration of the receptor-ligand complex at the KD? 1.0 Q2 10 15 20 25 30 35 40 45 50 55 60 0 0.5 40 mM O 25 mM O 30 mM O 10 mM 17.5 ml 5 [Ligand] (mm)
You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2
μM substrate. At this substrate concentration, you determine that the
Vo = 88.6 μmol mL-¹. s-¹. If the Vmax
833.3 mM s the KM is:
.
==
"
30.9 μΜ
10.4 μΜ
Ο 124.6 μΜ
234 μΜ
94.5 μM
Transcribed Image Text:You are evaluating the kinetics of an enzyme catalyzed reaction containing 5.5 μM total enzyme and 11.2 μM substrate. At this substrate concentration, you determine that the Vo = 88.6 μmol mL-¹. s-¹. If the Vmax 833.3 mM s the KM is: . == " 30.9 μΜ 10.4 μΜ Ο 124.6 μΜ 234 μΜ 94.5 μM
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 1 images

Blurred answer
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781319114671
Author:
Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:
W. H. Freeman
Lehninger Principles of Biochemistry
Lehninger Principles of Biochemistry
Biochemistry
ISBN:
9781464126116
Author:
David L. Nelson, Michael M. Cox
Publisher:
W. H. Freeman
Fundamentals of Biochemistry: Life at the Molecul…
Fundamentals of Biochemistry: Life at the Molecul…
Biochemistry
ISBN:
9781118918401
Author:
Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher:
WILEY
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305961135
Author:
Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher:
Cengage Learning
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Fundamentals of General, Organic, and Biological …
Fundamentals of General, Organic, and Biological …
Biochemistry
ISBN:
9780134015187
Author:
John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson
Publisher:
PEARSON