Examine the reaction below. Using the table of standard state free energies provided: A) Calculate the standard state free energy change for the reaction (in the direction shown). B) Under standard state conditions, will this reaction proceed to the LEFT(down) or RIGHT (up)? NH2 COMPOUND AG hydrolysis (kJ/mol) - 62 - 43 - 32 - 21 - 14 N Phosphoenolpyruvate Creatine phosphate CH,OH N. АТР он Glucose-1-phosphate Glucose-6-phosphate H OH ОРО H. ÓH ÓH OH NH2 N. N. CH,OH 0- Он OH OH H. OH OH OH o=-o o=-o
Examine the reaction below. Using the table of standard state free energies provided: A) Calculate the standard state free energy change for the reaction (in the direction shown). B) Under standard state conditions, will this reaction proceed to the LEFT(down) or RIGHT (up)? NH2 COMPOUND AG hydrolysis (kJ/mol) - 62 - 43 - 32 - 21 - 14 N Phosphoenolpyruvate Creatine phosphate CH,OH N. АТР он Glucose-1-phosphate Glucose-6-phosphate H OH ОРО H. ÓH ÓH OH NH2 N. N. CH,OH 0- Он OH OH H. OH OH OH o=-o o=-o
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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
Transcribed Image Text:**Transcription and Analysis of Reaction Using Standard State Free Energies**
**Reaction Analysis:**
The image presents a biochemical reaction involving ATP hydrolysis to ADP and the transfer of a phosphate group. The structures involved include a nucleotide and a sugar phosphate compound.
**Table of Standard State Free Energies:**
| Compound | ΔG°' (kJ/mol) hydrolysis |
|------------------------|--------------------------|
| Phosphoenolpyruvate | -62 |
| Creatine phosphate | -43 |
| ATP | -32 |
| Glucose-1-phosphate | -21 |
| Glucose-6-phosphate | -14 |
**Problems:**
**A) Calculate the standard state free energy change for the reaction (in the direction shown).**
1. Identify the compounds involved from the table:
- The reaction involves ATP hydrolysis to produce ADP and a phosphorylated sugar.
2. Use the given ΔG°' values:
- ATP hydrolysis: ΔG°' = -32 kJ/mol
3. Calculate the overall standard state free energy change using the provided data.
**B) Under standard state conditions, will this reaction proceed to the LEFT (down) or RIGHT (up)?**
1. Determine the directionality based on the ΔG°' value calculation:
- Negative ΔG°' indicates a reaction that proceeds spontaneously in the given direction (right/up).
- Positive ΔG°' would indicate the reverse direction.
**Graphical Explanation:**
- The reaction diagram shows ATP interacting with a sugar molecule, resulting in the transfer of a phosphate group.
- The diagram includes molecular structures illustrating the reactants' transformation to products upon phosphate transfer.
**Conclusion:**
Based on the standard free energy change from ATP hydrolysis being negative, this reaction is likely to proceed to the right under standard state conditions, releasing energy as the phosphate group is transferred. This demonstrates a typical biochemical energetics calculation used to predict reaction feasibility and direction.
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