27. The standard-state free energy of hydrolysis for acetyl phosphate in 1,3-Bisphosphoglycerate is 1,3-Bisphosphoglycerate+ H₂O 3-phosphoglycerate + Pi AG" -49.6 kJ/mol. Determine the free energy change for 1,3-Bisphosphoglycerate hydrolysis in a solution of 5.4 mM 3-phosphoglycerate, 0.0054 M inorganic phosphate, and 20 nM 1,3-Bisphosphoglycerate.

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